{"id":"c0745db2-eca6-5416-ba2c-76ae4686791e","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-cralbp-des1-isomer-yield","predicate":"increases_assay_yield_of","statement":"CRALBP coexpression increased 11-cis-retinol production approximately threefold in homogenates of cells stably expressing chicken DES1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b22a179a-36d7-5cf5-9318-f620f7dbb514","mechanism_event_label":"The binding protein shifted the measured output toward the visual-cycle alcohol.","subject":{"id":"fd923f7d-57da-58da-ad04-adb75072433f","slug":"rlbp1","display_name":"Cellular retinaldehyde-binding protein / CRALBP","entity_type_key":"protein"},"object":{"id":"4a978267-139b-518d-bdc6-e16cda6f5225","slug":"11-cis-retinol","display_name":"11-cis-retinol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b22a179a-36d7-5cf5-9318-f620f7dbb514","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-cralbp-des1-isomer-yield-event","event_type":"biochemical_relationship","label":"The binding protein shifted the measured output toward the visual-cycle alcohol.","description":"CRALBP coexpression increased 11-cis-retinol production approximately threefold in homogenates of cells stably expressing chicken DES1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fd923f7d-57da-58da-ad04-adb75072433f","slug":"rlbp1","display_name":"Cellular retinaldehyde-binding protein / CRALBP","entity_type_key":"protein"},"role":"coexpressed binding protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"743b0a10-1977-5b02-a116-1c53c6700b3d","slug":"degs1","display_name":"Dihydroceramide desaturase 1 / DES1","entity_type_key":"protein"},"role":"candidate isomerase","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4a978267-139b-518d-bdc6-e16cda6f5225","slug":"11-cis-retinol","display_name":"11-cis-retinol","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 5b","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chicken CRALBP transfection into stable DES1-expressing 293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"All-trans-retinol substrate; CRALBP plasmid versus empty-vector comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An assay yield effect does not prove physiological transport or human supplementation benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Gallus gallus proteins in Homo sapiens cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The binding protein shifted the measured output toward the visual-cycle alcohol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"cell homogenates","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"68294b33-4f22-5cd4-a67d-ecf66941e477","evidence_kind":"source_excerpt","locator":"Lines 573-586","start_line":573,"end_line":586,"excerpt":"### vae-cralbp-des1-isomer-yield\nCRALBP coexpression increased 11-cis-retinol production approximately threefold in homogenates of cells stably expressing chicken DES1.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The binding protein shifted the measured output toward the visual-cycle alcohol.\norganism: Gallus gallus proteins in Homo sapiens cells\ntissue_or_cell_type: cell homogenates\nexperimental_model: Chicken CRALBP transfection into stable DES1-expressing 293T cells\nlimitations: An assay yield effect does not prove physiological transport or human supplementation benefit.\nexposure: All-trans-retinol substrate; CRALBP plasmid versus empty-vector comparison.\ncross_nutrient: false\nevidence_location: Figure 5b\nnutrient: Vitamin A\n[vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114","model_system":"Chicken CRALBP transfection into stable DES1-expressing 293T cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"559cb4dc3f59519ecb0bc455dbcc73346fbf8e0c51e133f455781f51e4ed07ab","revision_id":"225fe4da-2d21-5c19-a92c-80a5f3cac8cb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}