Component

Allicin-evoked current through rat TRPV1 in HEK293 patches

Allicin-evoked current through rat TRPV1 in HEK293 patches. Interpret through the linked study species, preparation, exposure and measured endpoint.

5 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.

    Dithiothreitol / DTT → Allicin-evoked current through rat TRPV1 in HEK293 patches source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Allicin-activated channel before DTT prior exposure · Allicin Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Allicin-activated channel before DTT added · Dithiothreitol / DTT Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "DTT after allicin", "comparator": "Allicin-activated channel before DTT", "endpoint": "A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.", "effect_direction": "decrease", "combination": "joint", "conditions": [{"entity_slug": "dithiothreitol", "state": "added"}, {"entity_slug": "allicin", "state": "prior exposure"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Rat TRPV1 in HEK293; DTT addition after allicin exposure.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Experimental reducing agent, not an oral treatment recommendation.
    plain_language
    A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.
    primary_references
    A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056
    source_locator
    Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 73–73

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Rat TRPV1 in HEK293; DTT addition after allicin exposure. · source_derived_draft · unverified_draft

    **A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)
    Complete structured claim and evidence

  2. Allicin activated rat TRPV1 expressed in HEK293 membrane patches.

    Allicin → Allicin-evoked current through rat TRPV1 in HEK293 patches source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_contrast
    {"intervention": "Allicin exposure", "comparator": "Patch before allicin", "endpoint": "Allicin activated rat TRPV1 expressed in HEK293 membrane patches.", "effect_direction": "increase", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Rat TRPV1 construct in human HEK293 host cells; inside-out patch recordings.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.
    plain_language
    Allicin activated rat TRPV1 expressed in HEK293 membrane patches.
    primary_references
    A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056
    source_locator
    Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 73–73

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Rat TRPV1 construct in human HEK293 host cells; inside-out patch recordings. · source_derived_draft · unverified_draft

    **A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)
    Complete structured claim and evidence

  3. Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.

    Allicin → Allicin-evoked current through rat TRPV1 in HEK293 patches source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Cysteine-less construct at corresponding allicin exposure applied · Allicin Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Cysteine-less construct at corresponding allicin exposure expressed · Rat TRPV1 cysteine-less construct with C157 restored Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "Allicin applied to C157-restored construct", "comparator": "Cysteine-less construct at corresponding allicin exposure", "endpoint": "Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "rat-trpv1-c157-only", "state": "expressed"}, {"entity_slug": "allicin", "state": "applied"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Rat TRPV1 C157-only construct in HEK293; Figure 8.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.
    plain_language
    Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.
    primary_references
    A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056
    source_locator
    Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 73–73

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Rat TRPV1 C157-only construct in HEK293; Figure 8. · source_derived_draft · unverified_draft

    **A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)
    Complete structured claim and evidence

  4. The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.

    Allicin → Allicin-evoked current through rat TRPV1 in HEK293 patches source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Same mutant patch before allicin expressed · Rat TRPV1 C157A experimental construct Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Same mutant patch before allicin applied · Allicin Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "Allicin applied to rat-trpv1-c157a", "comparator": "Same mutant patch before allicin", "endpoint": "The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "rat-trpv1-c157a", "state": "expressed"}, {"entity_slug": "allicin", "state": "applied"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Rat channel variant expressed in HEK293; Figure 8 functional comparison.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    No response to allicin, not nonfunctional protein. Human homolog residue numbers are not silently assigned.
    plain_language
    The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.
    primary_references
    A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056
    source_locator
    Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 73–73

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Rat channel variant expressed in HEK293; Figure 8 functional comparison. · source_derived_draft · unverified_draft

    **A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)
    Complete structured claim and evidence

  5. The rat-trpv1-cysteine-less construct did not show allicin responsiveness while relevant capsaicin responses persisted.

    Allicin → Allicin-evoked current through rat TRPV1 in HEK293 patches source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.
    experimental_condition
    Same mutant patch before allicin applied · Allicin Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Same mutant patch before allicin expressed · Rat TRPV1 cysteine-less experimental construct Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "Allicin applied to rat-trpv1-cysteine-less", "comparator": "Same mutant patch before allicin", "endpoint": "The rat-trpv1-cysteine-less construct did not show allicin responsiveness while relevant capsaicin responses persisted.", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "rat-trpv1-cysteine-less", "state": "expressed"}, {"entity_slug": "allicin", "state": "applied"}]} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Rat channel variant expressed in HEK293; Figure 8 functional comparison.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    No response to allicin, not nonfunctional protein. Human homolog residue numbers are not silently assigned.
    plain_language
    The rat-trpv1-cysteine-less construct did not show allicin responsiveness while relevant capsaicin responses persisted.
    primary_references
    A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056
    source_locator
    Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.

    Allicin: detailed mechanisms of action (reviewed 5 October 2026) · lines 73–73

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Rat channel variant expressed in HEK293; Figure 8 functional comparison. · source_derived_draft · unverified_draft

    **A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.