{"id":"cc033af1-9dcb-52b8-a143-a0ef23e19286","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:trpv1-c157-restored","predicate":"increases_in_recorded_experiment","statement":"Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b89d6bd0-23be-58df-bb8e-a95b1af481c3","mechanism_event_label":"Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b89d6bd0-23be-58df-bb8e-a95b1af481c3","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:trpv1-c157-restored-event","event_type":"biochemical_relationship","label":"Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.","description":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin applied to C157-restored construct","sequence_order":0,"notes":""},{"entity":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"c49c7f6c-6d20-5efe-b242-7a7b3ac07bff","slug":"rat-trpv1-c157-only","display_name":"Rat TRPV1 cysteine-less construct with C157 restored","entity_type_key":"protein_state"},"role":"restored construct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"applied","comparator":"Cysteine-less construct at corresponding allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"expressed","comparator":"Cysteine-less construct at corresponding allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"rat-trpv1-c157-only","display_name":"Rat TRPV1 cysteine-less construct with C157 restored","entity_type_key":"protein_state"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin applied to C157-restored construct\", \"comparator\": \"Cysteine-less construct at corresponding allicin exposure\", \"endpoint\": \"Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"rat-trpv1-c157-only\", \"state\": \"expressed\"}, {\"entity_slug\": \"allicin\", \"state\": \"applied\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat TRPV1 C157-only construct in HEK293; Figure 8.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Restoring C157 to the cysteine-less rat TRPV1 construct restored responsiveness to allicin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a62d5f09-22c9-5a61-96d3-5ac3a9c0450e","evidence_kind":"source_excerpt","locator":"Lines 73-73","start_line":73,"end_line":73,"excerpt":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","model_system":"Rat TRPV1 C157-only construct in HEK293; Figure 8.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}