{"id":"a311dd6f-f19e-5fbf-9836-4280c26ab5de","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:trpv1-mutant-rat-trpv1-c157a","predicate":"no_detected_change_in_recorded_experiment","statement":"The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"3c9748e8-0944-5a18-884a-50aaac7fda41","mechanism_event_label":"The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c9748e8-0944-5a18-884a-50aaac7fda41","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:trpv1-mutant-rat-trpv1-c157a-event","event_type":"experimental_observation","label":"The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.","description":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin applied to rat-trpv1-c157a","sequence_order":0,"notes":""},{"entity":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"453fec0a-cf59-5492-8e5c-4c1a8e0bf6d5","slug":"rat-trpv1-c157a","display_name":"Rat TRPV1 C157A experimental construct","entity_type_key":"protein_state"},"role":"tested variant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"separate functional positive-control agonist","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"applied","comparator":"Same mutant patch before allicin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"expressed","comparator":"Same mutant patch before allicin","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"rat-trpv1-c157a","display_name":"Rat TRPV1 C157A experimental construct","entity_type_key":"protein_state"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin applied to rat-trpv1-c157a\", \"comparator\": \"Same mutant patch before allicin\", \"endpoint\": \"The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"rat-trpv1-c157a\", \"state\": \"expressed\"}, {\"entity_slug\": \"allicin\", \"state\": \"applied\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat channel variant expressed in HEK293; Figure 8 functional comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No response to allicin, not nonfunctional protein. Human homolog residue numbers are not silently assigned.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The rat-trpv1-c157a construct did not show allicin responsiveness while relevant capsaicin responses persisted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"270a7914-9e2f-5dc9-a47b-02e9de731de5","evidence_kind":"source_excerpt","locator":"Lines 73-73","start_line":73,"end_line":73,"excerpt":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","model_system":"Rat channel variant expressed in HEK293; Figure 8 functional comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}