{"id":"87350b52-2ce5-50a3-93eb-cfc76053e9e8","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:rat-trpv1-activation","predicate":"increases_in_recorded_experiment","statement":"Allicin activated rat TRPV1 expressed in HEK293 membrane patches.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"069120b7-3fa1-5582-a059-e3c553ec6cf5","mechanism_event_label":"Allicin activated rat TRPV1 expressed in HEK293 membrane patches.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"069120b7-3fa1-5582-a059-e3c553ec6cf5","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:rat-trpv1-activation-event","event_type":"biochemical_relationship","label":"Allicin activated rat TRPV1 expressed in HEK293 membrane patches.","description":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin exposure","sequence_order":0,"notes":""},{"entity":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"c03abe8d-abba-5d01-a999-caedc9a17941","slug":"rat-trpv1","display_name":"Rat transient receptor potential vanilloid 1 / Trpv1","entity_type_key":"protein"},"role":"expressed channel","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin exposure\", \"comparator\": \"Patch before allicin\", \"endpoint\": \"Allicin activated rat TRPV1 expressed in HEK293 membrane patches.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat TRPV1 construct in human HEK293 host cells; inside-out patch recordings.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Allicin activated rat TRPV1 expressed in HEK293 membrane patches.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 73-73; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f1bd856d-dcd3-518b-9b11-80dd1f22a20c","evidence_kind":"source_excerpt","locator":"Lines 73-73","start_line":73,"end_line":73,"excerpt":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","model_system":"Rat TRPV1 construct in human HEK293 host cells; inside-out patch recordings.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}