{"id":"db7da09f-02f6-5237-a849-f75f61581acb","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dtt-channel-reversal","predicate":"decreases_in_recorded_experiment","statement":"A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a4bdfde8-0321-5220-9380-9dc1b2ece572","mechanism_event_label":"A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.","subject":{"id":"d3f1dc28-551f-518b-863f-627b4ac2c5f3","slug":"dithiothreitol","display_name":"Dithiothreitol / DTT","entity_type_key":"small_molecule"},"object":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a4bdfde8-0321-5220-9380-9dc1b2ece572","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dtt-channel-reversal-event","event_type":"biochemical_relationship","label":"A reducing agent reversed allicin-evoked TRPV1 activation in the patch assay.","description":"**A tested TRPV1 cysteine.** In rat TRPV1 expressed in HEK293 cells, the C157A substitution eliminated allicin responsiveness; reinserting C157 into a cysteine-less construct restored it. Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d3f1dc28-551f-518b-863f-627b4ac2c5f3","slug":"dithiothreitol","display_name":"Dithiothreitol / DTT","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"DTT after allicin","sequence_order":0,"notes":""},{"entity":{"id":"9082cb48-04f0-5a40-acb5-b16ba1853dd4","slug":"rat-trpv1-allicin-current","display_name":"Allicin-evoked current through rat TRPV1 in HEK293 patches","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"c03abe8d-abba-5d01-a999-caedc9a17941","slug":"rat-trpv1","display_name":"Rat transient receptor potential vanilloid 1 / Trpv1","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"prior activator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. 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Capsaicin responsiveness persisted in the relevant mutant controls. A reducing agent reversed activation. This is direct functional evidence for a cysteine-dependent activation mechanism in that construct, not proof that every electrophile makes the same adduct or that the cell line makes the protein human. [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","model_system":"Rat TRPV1 in HEK293; DTT addition after allicin exposure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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