Component

Cardiomyocyte-specific mouse Dlat overexpression

Study-specific entity. Experimental conditions and evidence limits remain on each linked claim.

5 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

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How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

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What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.

    Cardiomyocyte-specific mouse Dlat overexpression → Mouse cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction
    duration
    Transgenic induction age and observation interval differ across passages; exact interval unresolved
    endpoint
    mouse-cardiac-fao
    evidence_location
    Oroboros respirometry; Figure 4I–K
    experimental_model
    Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated
    exposure
    mouse-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Substrate-supported respiratory capacity, not an intact-animal FAO flux. Methods call the substrate both octanoylcarnitine and palmitoyl-carnitine; retain the explicit reagent as reported, with ambiguity flagged.
    organism
    Mus musculus
    plain_language
    Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 35, "text_sha256": "28124428b0c4afe43ebd39f6ba3ab50da327de54267a02a3bdd81c53aa5c6743", "xml_element_id": "Par16"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 37, "text_sha256": "a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 88, "text_sha256": "da214c15c67429e79f372bef6347c2c7bc604429178f3a9db5651425732f37b4", "xml_element_id": "Par39"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 353–368

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated · source_derived_draft · unverified_draft

    Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated compartment: Cardiac tissue / mitochondria dose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction duration: Transgenic induction age and observation interval differ across passages; exact interval unresolved primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Oroboros respirometry; Figure 4I–K endpoint: mouse-cardiac-fao exposure: mouse-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Substrate-supported respiratory capacity, not an intact-animal FAO flux. Methods call the substrate both octanoylcarnitine and palmitoyl-carnitine; retain the explicit reagent as reported, with ambiguity flagged. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 35, "text_sha256": "28124428b0c4afe43ebd39f6ba3ab50da327de54267a02a3bdd81c53aa5c6743", "xml_element_id": "Par16"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 37, "text_sha256": "a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 88, "text_sha256": "da214c15c67429e79f372bef6347c2c7bc604429178f3a9db5651425732f37b4", "xml_element_id": "Par39"}] plain_language: Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations. sample_size: 4 biological replicates
    Complete structured claim and evidence

  2. Dlat-transgenic mice developed the reported diastolic-impairment phenotype while systolic function was preserved during the observation period.

    Cardiomyocyte-specific mouse Dlat overexpression → Mouse cardiac diastolic impairment source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction
    duration
    Transgenic induction age and observation interval differ across passages; exact interval unresolved
    endpoint
    mouse-cardiac-diastolic-impairment
    evidence_location
    Figure 4M–O; Supplementary Figure S18
    experimental_model
    Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated
    exposure
    mouse-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Main-text E/A and E/e-prime directions disagree with Figure 4 and source-data columns. The data show increased ratios; the erroneous directional prose is not used as an independent finding. Transgenic age arithmetic also requires clarification.
    organism
    Mus musculus
    plain_language
    Dlat-transgenic mice developed the reported diastolic-impairment phenotype while systolic function was preserved during the observation period.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 37, "text_sha256": "a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM4_ESM.xlsx", "location": "figure4; E/A rows 23-32, E/e-prime rows 41-50", "sha256": "7fc506fec970a4b7e9b2f370695d82b36020de2e77a0a26112066688d52606f5"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    8 per echocardiographic group
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 388–403

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated · source_derived_draft · unverified_draft

    Dlat-transgenic mice developed the reported diastolic-impairment phenotype while systolic function was preserved during the observation period. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated compartment: Cardiac tissue / mitochondria dose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction duration: Transgenic induction age and observation interval differ across passages; exact interval unresolved primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 4M–O; Supplementary Figure S18 endpoint: mouse-cardiac-diastolic-impairment exposure: mouse-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Main-text E/A and E/e-prime directions disagree with Figure 4 and source-data columns. The data show increased ratios; the erroneous directional prose is not used as an independent finding. Transgenic age arithmetic also requires clarification. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 37, "text_sha256": "a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM4_ESM.xlsx", "location": "figure4; E/A rows 23-32, E/e-prime rows 41-50", "sha256": "7fc506fec970a4b7e9b2f370695d82b36020de2e77a0a26112066688d52606f5"}] plain_language: Dlat-transgenic mice developed the reported diastolic-impairment phenotype while systolic function was preserved during the observation period. sample_size: 8 per echocardiographic group
    Complete structured claim and evidence

  3. Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.

    Cardiomyocyte-specific mouse Dlat overexpression → Mouse cardiac HADHA lysine acetylation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction
    duration
    Transgenic induction age and observation interval differ across passages; exact interval unresolved
    endpoint
    mouse-hadha-acetylation
    evidence_location
    HADHA IP/immunoblot; Figure 6I
    experimental_model
    Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated
    exposure
    mouse-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.
    organism
    Mus musculus
    plain_language
    Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 335–350

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated · source_derived_draft · unverified_draft

    Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated compartment: Cardiac tissue / mitochondria dose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction duration: Transgenic induction age and observation interval differ across passages; exact interval unresolved primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: HADHA IP/immunoblot; Figure 6I endpoint: mouse-hadha-acetylation exposure: mouse-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}] plain_language: Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance. sample_size: 4 biological replicates
    Complete structured claim and evidence

  4. Dlat-transgenic mouse hearts showed increased DAGs, ceramides and long-chain acylcarnitines in lipidomic measurements.

    Cardiomyocyte-specific mouse Dlat overexpression → Mouse cardiac DAG, ceramide and acylcarnitine measurements source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction
    duration
    Transgenic induction age and observation interval differ across passages; exact interval unresolved
    endpoint
    mouse-cardiac-lipotoxic-lipids
    evidence_location
    Supplementary Figure S17; mass-spectrometry lipidomics
    experimental_model
    Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated
    exposure
    mouse-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. These are measured associations after overexpression; the individual lipid species were not independently shown to mediate dysfunction in this paper.
    organism
    Mus musculus
    plain_language
    Dlat-transgenic mouse hearts showed increased DAGs, ceramides and long-chain acylcarnitines in lipidomic measurements.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 100, "text_sha256": "98a990aba7d1dc2debe7310603573da4dbe8b4ba17bd425ae6f6807b1f393ac8", "xml_element_id": "Par45"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 371–385

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated · source_derived_draft · unverified_draft

    Dlat-transgenic mouse hearts showed increased DAGs, ceramides and long-chain acylcarnitines in lipidomic measurements. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated compartment: Cardiac tissue / mitochondria dose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction duration: Transgenic induction age and observation interval differ across passages; exact interval unresolved primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Supplementary Figure S17; mass-spectrometry lipidomics endpoint: mouse-cardiac-lipotoxic-lipids exposure: mouse-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. These are measured associations after overexpression; the individual lipid species were not independently shown to mediate dysfunction in this paper. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 38, "text_sha256": "e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a", "xml_element_id": "Par17"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 100, "text_sha256": "98a990aba7d1dc2debe7310603573da4dbe8b4ba17bd425ae6f6807b1f393ac8", "xml_element_id": "Par45"}] plain_language: Dlat-transgenic mouse hearts showed increased DAGs, ceramides and long-chain acylcarnitines in lipidomic measurements.
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.

    Cardiomyocyte-targeted mouse HADHA overexpression → Mouse cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Cardiac tissue / mitochondria
    dose
    Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction
    duration
    Transgenic induction age and observation interval differ across passages; exact interval unresolved
    endpoint
    mouse-cardiac-fao
    evidence_location
    Supplementary Figure S21
    experimental_model
    Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated
    exposure
    mouse-hadha-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Wild-type HADHA overexpression, not a K728R mouse rescue; increased enzyme abundance does not isolate the acetylation-site mechanism.
    organism
    Mus musculus
    plain_language
    AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 52, "text_sha256": "74297778077d895148e1cc0c9819297c22c274b0ec6fdce0483ce8ba94ab58b4", "xml_element_id": "Par21"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Cardiac tissue and cardiomyocytes

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 477–492

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated · source_derived_draft · unverified_draft

    AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated compartment: Cardiac tissue / mitochondria dose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction duration: Transgenic induction age and observation interval differ across passages; exact interval unresolved primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Supplementary Figure S21 endpoint: mouse-cardiac-fao exposure: mouse-hadha-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Wild-type HADHA overexpression, not a K728R mouse rescue; increased enzyme abundance does not isolate the acetylation-site mechanism. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 52, "text_sha256": "74297778077d895148e1cc0c9819297c22c274b0ec6fdce0483ce8ba94ab58b4", "xml_element_id": "Par21"}] plain_language: AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts. sample_size: 4 biological replicates
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards