{"id":"de34924d-0ae9-5fb0-b881-2eb6fc2d276f","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-hadha-acetylation","predicate":"increases","statement":"Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"1f09e6a5-f20d-5931-94bb-a968c7726fde","mechanism_event_label":"mouse dlat hadha acetylation","subject":{"id":"64396364-a042-5b06-bc06-6e53c2b39109","slug":"mouse-dlat-overexpression","display_name":"Cardiomyocyte-specific mouse Dlat overexpression","entity_type_key":"protein_state"},"object":{"id":"7b7064d1-87e4-5fbd-9be1-0f3c37e58c4e","slug":"mouse-hadha-acetylation","display_name":"Mouse cardiac HADHA lysine acetylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"1f09e6a5-f20d-5931-94bb-a968c7726fde","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-hadha-acetylation","event_type":"increases","label":"mouse dlat hadha acetylation","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64396364-a042-5b06-bc06-6e53c2b39109","slug":"mouse-dlat-overexpression","display_name":"Cardiomyocyte-specific mouse Dlat overexpression","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7b7064d1-87e4-5fbd-9be1-0f3c37e58c4e","slug":"mouse-hadha-acetylation","display_name":"Mouse cardiac HADHA lysine acetylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0c45e35a-9af8-52e0-ac32-fde170ef9158","slug":"mouse-hadha","display_name":"Mouse mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Cardiac tissue / mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Transgenic induction age and observation interval differ across passages; exact interval unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"mouse-hadha-acetylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"HADHA IP/immunoblot; Figure 6I","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"mouse-dlat-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cardiac tissue and cardiomyocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"00b58cbb-84f4-57b0-845f-998943538151","evidence_kind":"source_excerpt","locator":"Lines 335-350","start_line":335,"end_line":350,"excerpt":"Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.\norganism: Mus musculus\ntissue_or_cell_type: Cardiac tissue and cardiomyocytes\nexperimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated\ncompartment: Cardiac tissue / mitochondria\ndose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction\nduration: Transgenic induction age and observation interval differ across passages; exact interval unresolved\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: HADHA IP/immunoblot; Figure 6I\nendpoint: mouse-hadha-acetylation\nexposure: mouse-dlat-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]\nplain_language: Cardiac Dlat overexpression increased HADHA acetylation without a corresponding significant change in HADHA abundance.\nsample_size: 4 biological replicates","model_system":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}