{"id":"c4d8601b-af9d-52d5-80c7-5fc7e6bfb04d","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-hadha-rescue","predicate":"increases","statement":"AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d5249416-cbd3-5900-a194-323cfa99dd07","mechanism_event_label":"mouse hadha rescue","subject":{"id":"9d31720a-fb23-52b9-b129-0d69357ab8ef","slug":"mouse-hadha-overexpression","display_name":"Cardiomyocyte-targeted mouse HADHA overexpression","entity_type_key":"protein_state"},"object":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d5249416-cbd3-5900-a194-323cfa99dd07","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-hadha-rescue","event_type":"increases","label":"mouse hadha rescue","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9d31720a-fb23-52b9-b129-0d69357ab8ef","slug":"mouse-hadha-overexpression","display_name":"Cardiomyocyte-targeted mouse HADHA overexpression","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"64396364-a042-5b06-bc06-6e53c2b39109","slug":"mouse-dlat-overexpression","display_name":"Cardiomyocyte-specific mouse Dlat overexpression","entity_type_key":"protein_state"},"role":"experimental background","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0c45e35a-9af8-52e0-ac32-fde170ef9158","slug":"mouse-hadha","display_name":"Mouse mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"role":"overexpressed protein","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Cardiac tissue / mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Transgenic induction age and observation interval differ across passages; exact interval unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"mouse-cardiac-fao","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Supplementary Figure S21","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"mouse-hadha-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Wild-type HADHA overexpression, not a K728R mouse rescue; increased enzyme abundance does not isolate the acetylation-site mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 52, \"text_sha256\": \"74297778077d895148e1cc0c9819297c22c274b0ec6fdce0483ce8ba94ab58b4\", \"xml_element_id\": \"Par21\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cardiac tissue and cardiomyocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6b9e3cd-8ab2-52ef-82a9-e7398af32dbf","evidence_kind":"source_excerpt","locator":"Lines 477-492","start_line":477,"end_line":492,"excerpt":"AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.\norganism: Mus musculus\ntissue_or_cell_type: Cardiac tissue and cardiomyocytes\nexperimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated\ncompartment: Cardiac tissue / mitochondria\ndose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction\nduration: Transgenic induction age and observation interval differ across passages; exact interval unresolved\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Supplementary Figure S21\nendpoint: mouse-cardiac-fao\nexposure: mouse-hadha-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Wild-type HADHA overexpression, not a K728R mouse rescue; increased enzyme abundance does not isolate the acetylation-site mechanism.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 52, \"text_sha256\": \"74297778077d895148e1cc0c9819297c22c274b0ec6fdce0483ce8ba94ab58b4\", \"xml_element_id\": \"Par21\"}]\nplain_language: AAV9-mediated HADHA overexpression restored FAO-associated respiration in Dlat-transgenic mouse hearts.\nsample_size: 4 biological replicates","model_system":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}