{"id":"bdb8c67d-4d43-5758-b6ca-d411b050190d","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-fao","predicate":"reduces","statement":"Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0540c161-2c66-5573-8d66-1cbe587d555f","mechanism_event_label":"mouse dlat fao","subject":{"id":"64396364-a042-5b06-bc06-6e53c2b39109","slug":"mouse-dlat-overexpression","display_name":"Cardiomyocyte-specific mouse Dlat overexpression","entity_type_key":"protein_state"},"object":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0540c161-2c66-5573-8d66-1cbe587d555f","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:mouse-dlat-fao","event_type":"reduces","label":"mouse dlat fao","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64396364-a042-5b06-bc06-6e53c2b39109","slug":"mouse-dlat-overexpression","display_name":"Cardiomyocyte-specific mouse Dlat overexpression","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4277e9b5-6f0b-55b4-89b1-3201b6182f04","slug":"mouse-cardiac-fao","display_name":"Mouse cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Cardiac tissue / mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Transgenic induction age and observation interval differ across passages; exact interval unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"mouse-cardiac-fao","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Oroboros respirometry; Figure 4I–K","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"mouse-dlat-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Substrate-supported respiratory capacity, not an intact-animal FAO flux. Methods call the substrate both octanoylcarnitine and palmitoyl-carnitine; retain the explicit reagent as reported, with ambiguity flagged.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 35, \"text_sha256\": \"28124428b0c4afe43ebd39f6ba3ab50da327de54267a02a3bdd81c53aa5c6743\", \"xml_element_id\": \"Par16\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 37, \"text_sha256\": \"a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 38, \"text_sha256\": \"e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a\", \"xml_element_id\": \"Par17\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 88, \"text_sha256\": \"da214c15c67429e79f372bef6347c2c7bc604429178f3a9db5651425732f37b4\", \"xml_element_id\": \"Par39\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cardiac tissue and cardiomyocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ed660a2e-d8ce-5077-9a17-4c23d97c0c71","evidence_kind":"source_excerpt","locator":"Lines 353-368","start_line":353,"end_line":368,"excerpt":"Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.\norganism: Mus musculus\ntissue_or_cell_type: Cardiac tissue and cardiomyocytes\nexperimental_model: Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated\ncompartment: Cardiac tissue / mitochondria\ndose: Tamoxifen-induced genetic overexpression; vector/induction dose not established in this extraction\nduration: Transgenic induction age and observation interval differ across passages; exact interval unresolved\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Oroboros respirometry; Figure 4I–K\nendpoint: mouse-cardiac-fao\nexposure: mouse-dlat-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Substrate-supported respiratory capacity, not an intact-animal FAO flux. Methods call the substrate both octanoylcarnitine and palmitoyl-carnitine; retain the explicit reagent as reported, with ambiguity flagged.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 35, \"text_sha256\": \"28124428b0c4afe43ebd39f6ba3ab50da327de54267a02a3bdd81c53aa5c6743\", \"xml_element_id\": \"Par16\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 37, \"text_sha256\": \"a3d0b7ba03c94c00e5cd07993ebd789dd554b51fafd62ec7937c72175c3c2a60\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 38, \"text_sha256\": \"e2abb7e90e9e5c62be80dfa1edc1640c63d0d2e3e053cc6c5fb631e57372200a\", \"xml_element_id\": \"Par17\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 88, \"text_sha256\": \"da214c15c67429e79f372bef6347c2c7bc604429178f3a9db5651425732f37b4\", \"xml_element_id\": \"Par39\"}]\nplain_language: Cardiac-specific Dlat overexpression reduced octanoylcarnitine-supported respiration in mouse heart preparations.\nsample_size: 4 biological replicates","model_system":"Conditional cardiac Dlat-transgenic mice; HADHA AAV9 rescue where stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}