Component

GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved

Context-specific entity; species, compartment and exposure are stated on each claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Spermidine permeated activated GluN1/GluN2A and GluN1/GluN2B channels; reported Km values were 2.2 and 2.7 mM.

    GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved → Spermidine source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions.
    limitations
    Millimolar experimental kinetics are not physiological brain concentrations.
    nutrient_topic
    Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
    plain_language
    A receptor channel can also provide an entry route.
    primary_references
    Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081

    Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 446–452

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions. · source_derived_draft · unverified_draft

    ## spermidine-nmda-entry A receptor channel can also provide an entry route. Spermidine permeated activated GluN1/GluN2A and GluN1/GluN2B channels; reported Km values were 2.2 and 2.7 mM. Model: Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions. Limitations: Millimolar experimental kinetics are not physiological brain concentrations. Evidence access: Primary abstract Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081
    Complete structured claim and evidence

What acts on it

  1. Calcium inhibited spermidine uptake through the tested NMDA receptors.

    Calcium ion → GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Receptor uptake assays in defined ionic solutions.
    limitations
    No dietary calcium–spermidine antagonism established.
    nutrient_topic
    Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
    plain_language
    Ions and polyamines can affect each other’s channel passage.
    primary_references
    Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081

    Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 462–468

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Receptor uptake assays in defined ionic solutions. · source_derived_draft · unverified_draft

    ## spermidine-nmda-calcium Ions and polyamines can affect each other’s channel passage. Calcium inhibited spermidine uptake through the tested NMDA receptors. Model: Receptor uptake assays in defined ionic solutions. Limitations: No dietary calcium–spermidine antagonism established. Evidence access: Primary abstract Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081
    Complete structured claim and evidence

  2. Spermidine uptake through the tested NMDA receptors required glycine and glutamate.

    Glycine → GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Heterologous receptor transport assays.
    limitations
    Requirement is for receptor activation, not evidence for an oral supplement combination.
    nutrient_topic
    Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
    plain_language
    Other amino acids gate this entry route.
    primary_references
    Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081

    Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 454–460

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Heterologous receptor transport assays. · source_derived_draft · unverified_draft

    ## spermidine-nmda-ligands Other amino acids gate this entry route. Spermidine uptake through the tested NMDA receptors required glycine and glutamate. Model: Heterologous receptor transport assays. Limitations: Requirement is for receptor activation, not evidence for an oral supplement combination. Evidence access: Primary abstract Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081
    Complete structured claim and evidence

  3. Spermidine and calcium influx persisted under magnesium conditions that strongly inhibited sodium influx.

    Mg2+ → GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Defined receptor assay solutions.
    limitations
    Voltage and ionic conditions are essential; no general claim that spermidine bypasses all NMDA magnesium block.
    nutrient_topic
    Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit. · Spermidine
    plain_language
    A magnesium block is not identical for every permeating species.
    primary_references
    Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081

    Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19) · lines 470–476

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Defined receptor assay solutions. · source_derived_draft · unverified_draft

    ## spermidine-nmda-magnesium A magnesium block is not identical for every permeating species. Spermidine and calcium influx persisted under magnesium conditions that strongly inhibited sodium influx. Model: Defined receptor assay solutions. Limitations: Voltage and ionic conditions are essential; no general claim that spermidine bypasses all NMDA magnesium block. Evidence access: Primary abstract Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards