{"id":"09b4e0a6-f30e-55cd-99b6-34019ff2bb70","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-ligands","predicate":"with_glutamate_enables","statement":"Spermidine uptake through the tested NMDA receptors required glycine and glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d3251e8b-51ef-5576-8e83-c4da7aae50ea","mechanism_event_label":"Other amino acids gate this entry route.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d3251e8b-51ef-5576-8e83-c4da7aae50ea","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-ligands-event","event_type":"observed_relationship","label":"Other amino acids gate this entry route.","description":"Spermidine uptake through the tested NMDA receptors required glycine and glutamate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Heterologous receptor transport assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Requirement is for receptor activation, not evidence for an oral supplement combination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Other amino acids gate this entry route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fdf9aa5f-bdc3-5395-9055-be3449178972","evidence_kind":"source_excerpt","locator":"Lines 454-460","start_line":454,"end_line":460,"excerpt":"## spermidine-nmda-ligands\nOther amino acids gate this entry route.\nSpermidine uptake through the tested NMDA receptors required glycine and glutamate.\nModel: Heterologous receptor transport assays.\nLimitations: Requirement is for receptor activation, not evidence for an oral supplement combination.\nEvidence access: Primary abstract\nSpermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","model_system":"Heterologous receptor transport assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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