{"id":"48b13900-9635-5b19-919e-5fdfed6450fb","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-entry","predicate":"permits_entry_of","statement":"Spermidine permeated activated GluN1/GluN2A and GluN1/GluN2B channels; reported Km values were 2.2 and 2.7 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2e59fec1-dbb4-5b05-96f3-ef5f1b0705ab","mechanism_event_label":"A receptor channel can also provide an entry route.","subject":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"object":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2e59fec1-dbb4-5b05-96f3-ef5f1b0705ab","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-entry-event","event_type":"observed_relationship","label":"A receptor channel can also provide an entry route.","description":"Spermidine permeated activated GluN1/GluN2A and GluN1/GluN2B channels; reported Km values were 2.2 and 2.7 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar experimental kinetics are not physiological brain concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A receptor channel can also provide an entry route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9fc2afb7-e32d-5f6e-8f35-81ac97c41d3c","evidence_kind":"source_excerpt","locator":"Lines 446-452","start_line":446,"end_line":452,"excerpt":"## spermidine-nmda-entry\nA receptor channel can also provide an entry route.\nSpermidine permeated activated GluN1/GluN2A and GluN1/GluN2B channels; reported Km values were 2.2 and 2.7 mM.\nModel: Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions.\nLimitations: Millimolar experimental kinetics are not physiological brain concentrations.\nEvidence access: Primary abstract\nSpermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","model_system":"Receptors expressed in Xenopus oocytes and HEK293 cells; isotonic extracellular solutions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}