{"id":"8cf20cdc-663c-5ac5-8424-f28da08c9892","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-calcium","predicate":"inhibits_spermidine_uptake_through","statement":"Calcium inhibited spermidine uptake through the tested NMDA receptors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cb995db1-54f3-5721-81d6-8793456c4b4a","mechanism_event_label":"Ions and polyamines can affect each other’s channel passage.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cb995db1-54f3-5721-81d6-8793456c4b4a","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-calcium-event","event_type":"observed_relationship","label":"Ions and polyamines can affect each other’s channel passage.","description":"Calcium inhibited spermidine uptake through the tested NMDA receptors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor uptake assays in defined ionic solutions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No dietary calcium–spermidine antagonism established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Ions and polyamines can affect each other’s channel passage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"83debb54-3fe3-5eb8-9069-3b21327335cc","evidence_kind":"source_excerpt","locator":"Lines 462-468","start_line":462,"end_line":468,"excerpt":"## spermidine-nmda-calcium\nIons and polyamines can affect each other’s channel passage.\nCalcium inhibited spermidine uptake through the tested NMDA receptors.\nModel: Receptor uptake assays in defined ionic solutions.\nLimitations: No dietary calcium–spermidine antagonism established.\nEvidence access: Primary abstract\nSpermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","model_system":"Receptor uptake assays in defined ionic solutions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}