{"id":"500bb7ea-9b5c-54a9-9898-a4f1da526587","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-magnesium","predicate":"differentially_limits_permeation","statement":"Spermidine and calcium influx persisted under magnesium conditions that strongly inhibited sodium influx.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1118f720-bbf9-572e-9d1c-07074a4f252f","mechanism_event_label":"A magnesium block is not identical for every permeating species.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1118f720-bbf9-572e-9d1c-07074a4f252f","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-nmda-magnesium-event","event_type":"observed_relationship","label":"A magnesium block is not identical for every permeating species.","description":"Spermidine and calcium influx persisted under magnesium conditions that strongly inhibited sodium influx.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44642d11-343b-5d4b-9a43-abbb2bcf326f","slug":"glun1-glun2a-b-permeation-assay","display_name":"GluN1/GluN2A or GluN1/GluN2B NMDA receptor permeation assay; construct species unresolved","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Defined receptor assay solutions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Voltage and ionic conditions are essential; no general claim that spermidine bypasses all NMDA magnesium block.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A magnesium block is not identical for every permeating species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a235e5d1-a2c7-53fd-bcba-d9f0241b61b7","evidence_kind":"source_excerpt","locator":"Lines 470-476","start_line":470,"end_line":476,"excerpt":"## spermidine-nmda-magnesium\nA magnesium block is not identical for every permeating species.\nSpermidine and calcium influx persisted under magnesium conditions that strongly inhibited sodium influx.\nModel: Defined receptor assay solutions.\nLimitations: Voltage and ionic conditions are essential; no general claim that spermidine bypasses all NMDA magnesium block.\nEvidence access: Primary abstract\nSpermidine and Ca(2+), but not Na(+), can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg(2+). · 2015 · https://pubmed.ncbi.nlm.nih.gov/26086092/ · DOI 10.1016/j.bbrc.2015.06.081","model_system":"Defined receptor assay solutions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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