Component

7-Hydroxycoumarin / umbelliferone

7-Hydroxycoumarin / umbelliferone. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. 7-Hydroxycoumarin also produced dose- and time-dependent reversible growth inhibition.

    7-Hydroxycoumarin / umbelliferone → Proliferation of study-specified human malignant cell lines source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"}
    experimental_model
    Dose/time exposure and washout in malignant cell lines
    exposure
    Coumarin and 7-hydroxycoumarin in cell culture
    limitations
    Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human cell lines
    plain_language
    The metabolite had its own measured activity.
    primary_references
    [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    tissue_or_cell_type
    Study panel including HL-60, A549, LNCaP and other tumor lines

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 423–434

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Dose/time exposure and washout in malignant cell lines · source_derived_draft · unverified_draft

    ### coumarin-metabolite-cytostasis 7-Hydroxycoumarin also produced dose- and time-dependent reversible growth inhibition. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The metabolite had its own measured activity. organism: Human cell lines tissue_or_cell_type: Study panel including HL-60, A549, LNCaP and other tumor lines experimental_model: Dose/time exposure and washout in malignant cell lines limitations: Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract. exposure: Coumarin and 7-hydroxycoumarin in cell culture evidence_span: {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"} [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    Complete structured claim and evidence

What acts on it

  1. Human CYP2A6 catalyzed conversion of coumarin to 7-hydroxycoumarin.

    Human cytochrome P450 2A6 / CYP2A6 → 7-Hydroxycoumarin / umbelliferone source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/15665333.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "79f42118f337ffe818b92138941685126173355131f8e12ca7cae9a437800a5b", "start_char": 0, "end_char": 1689, "text_sha256": "79f42118f337ffe818b92138941685126173355131f8e12ca7cae9a437800a5b"}
    experimental_model
    Purified human CYP2A6 kinetic experiments
    exposure
    Coumarin oxidation; stopped-flow and steady-state kinetics, selected measures at 23 C
    limitations
    Biochemical rates do not measure whole-body clearance; accessory b5 species is not specified in the abstract.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human CYP2A6 with reconstituted accessory proteins
    plain_language
    CYP2A6 adds an oxygen-containing group to coumarin.
    primary_references
    [coumarin-p15665333] Kinetic analysis of oxidation of coumarins by human cytochrome P450 2A6. (2005). https://pubmed.ncbi.nlm.nih.gov/15665333/ DOI: 10.1074/jbc.m411019200
    tissue_or_cell_type
    Reconstituted enzyme system

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 137–148

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purified human CYP2A6 kinetic experiments · source_derived_draft · unverified_draft

    ### coumarin-2a6-7oh Human CYP2A6 catalyzed conversion of coumarin to 7-hydroxycoumarin. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: CYP2A6 adds an oxygen-containing group to coumarin. organism: Human CYP2A6 with reconstituted accessory proteins tissue_or_cell_type: Reconstituted enzyme system experimental_model: Purified human CYP2A6 kinetic experiments limitations: Biochemical rates do not measure whole-body clearance; accessory b5 species is not specified in the abstract. exposure: Coumarin oxidation; stopped-flow and steady-state kinetics, selected measures at 23 C evidence_span: {"source_cache": "artifacts/coumarin-research/15665333.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "79f42118f337ffe818b92138941685126173355131f8e12ca7cae9a437800a5b", "start_char": 0, "end_char": 1689, "text_sha256": "79f42118f337ffe818b92138941685126173355131f8e12ca7cae9a437800a5b"} [coumarin-p15665333] Kinetic analysis of oxidation of coumarins by human cytochrome P450 2A6. (2005). https://pubmed.ncbi.nlm.nih.gov/15665333/ DOI: 10.1074/jbc.m411019200
    Complete structured claim and evidence

  2. Mean 7-hydroxycoumarin formation was 1230 of 1420 pmol/min/mg total polar products in the 12-sample human microsomal panel.

    Coumarin → 7-Hydroxycoumarin / umbelliferone source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/7839702.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909", "start_char": 0, "end_char": 1457, "text_sha256": "ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909"}
    experimental_model
    Radiolabeled substrate metabolism in 12 human liver microsomal samples
    exposure
    50 micromolar [3-14C]coumarin
    limitations
    Donor-panel correlations do not assign enzyme causality; older grouping of 3-hydroxylation products is not adopted as an epoxide mechanism.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human
    plain_language
    Most measured polar product was 7-hydroxycoumarin in this experiment.
    primary_references
    [coumarin-p7839702] Metabolism of [3-14C] coumarin by human liver microsomes. (1994). https://pubmed.ncbi.nlm.nih.gov/7839702/ DOI: 10.3109/00498259409043279
    tissue_or_cell_type
    Liver microsomes

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 189–200

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Radiolabeled substrate metabolism in 12 human liver microsomal samples · source_derived_draft · unverified_draft

    ### coumarin-microsomal-main-product Mean 7-hydroxycoumarin formation was 1230 of 1420 pmol/min/mg total polar products in the 12-sample human microsomal panel. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Most measured polar product was 7-hydroxycoumarin in this experiment. organism: Human tissue_or_cell_type: Liver microsomes experimental_model: Radiolabeled substrate metabolism in 12 human liver microsomal samples limitations: Donor-panel correlations do not assign enzyme causality; older grouping of 3-hydroxylation products is not adopted as an epoxide mechanism. exposure: 50 micromolar [3-14C]coumarin evidence_span: {"source_cache": "artifacts/coumarin-research/7839702.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909", "start_char": 0, "end_char": 1457, "text_sha256": "ca24fcb6d3561aa6605b2a53ff463aa6e2ca085d785d1f77615498580d84e909"} [coumarin-p7839702] Metabolism of [3-14C] coumarin by human liver microsomes. (1994). https://pubmed.ncbi.nlm.nih.gov/7839702/ DOI: 10.3109/00498259409043279
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. Bovine liver UGT preparation converted 7-hydroxycoumarin and UDP-glucuronic acid into its glucuronide.

    Bovine liver UDP-glucuronosyltransferase preparation; isoforms unresolved → 7-Hydroxycoumarin glucuronide source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/8877866.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d", "start_char": 0, "end_char": 2114, "text_sha256": "8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d"}
    experimental_model
    Enzyme assay and HPLC metabolite identification
    exposure
    7-Hydroxycoumarin plus UDP-glucuronic acid
    limitations
    Bovine preparation does not identify a dominant human UGT isoform.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Bovine
    plain_language
    A sugar-acid donor enables the conjugation step.
    primary_references
    [coumarin-p8877866] Analysis of the glucuronidation of 7-hydroxycoumarin by HPLC. (1996). https://pubmed.ncbi.nlm.nih.gov/8877866/ DOI: 10.1016/0731-7085(96)01801-8
    tissue_or_cell_type
    Liver homogenate/crude UGT preparation

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 540–551

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Enzyme assay and HPLC metabolite identification · source_derived_draft · unverified_draft

    ### coumarin-ugt-conjugation Bovine liver UGT preparation converted 7-hydroxycoumarin and UDP-glucuronic acid into its glucuronide. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: A sugar-acid donor enables the conjugation step. organism: Bovine tissue_or_cell_type: Liver homogenate/crude UGT preparation experimental_model: Enzyme assay and HPLC metabolite identification limitations: Bovine preparation does not identify a dominant human UGT isoform. exposure: 7-Hydroxycoumarin plus UDP-glucuronic acid evidence_span: {"source_cache": "artifacts/coumarin-research/8877866.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d", "start_char": 0, "end_char": 2114, "text_sha256": "8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d"} [coumarin-p8877866] Analysis of the glucuronidation of 7-hydroxycoumarin by HPLC. (1996). https://pubmed.ncbi.nlm.nih.gov/8877866/ DOI: 10.1016/0731-7085(96)01801-8
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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