{"id":"b2cfa71e-b838-5e80-9ae8-3b1987c236da","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-ugt-conjugation","predicate":"catalyzes_formation_of","statement":"Bovine liver UGT preparation converted 7-hydroxycoumarin and UDP-glucuronic acid into its glucuronide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d49aefeb-0511-508c-a548-8eed76d20e98","mechanism_event_label":"A sugar-acid donor enables the conjugation step.","subject":{"id":"d26361df-ca51-5eea-971b-2f1b2694427e","slug":"coumarin-bovine-ugt-preparation","display_name":"Bovine liver UDP-glucuronosyltransferase preparation; isoforms unresolved","entity_type_key":"protein_family"},"object":{"id":"b0f1cd5a-af15-52b4-a65e-a8c43a3bd886","slug":"7-hydroxycoumarin-glucuronide","display_name":"7-Hydroxycoumarin glucuronide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"d49aefeb-0511-508c-a548-8eed76d20e98","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-ugt-conjugation-event","event_type":"biochemical_relationship","label":"A sugar-acid donor enables the conjugation step.","description":"Bovine liver UGT preparation converted 7-hydroxycoumarin and UDP-glucuronic acid into its glucuronide.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b1e49924-a54c-59dd-9de3-59f8bbc4be2b","slug":"7-hydroxycoumarin","display_name":"7-Hydroxycoumarin / umbelliferone","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fea4f3f0-7c05-59a2-9332-445b9d4f8fb0","slug":"udp-glucuronic-acid","display_name":"UDP-glucuronic acid","entity_type_key":"small_molecule"},"role":"cosubstrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d26361df-ca51-5eea-971b-2f1b2694427e","slug":"coumarin-bovine-ugt-preparation","display_name":"Bovine liver UDP-glucuronosyltransferase preparation; isoforms unresolved","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b0f1cd5a-af15-52b4-a65e-a8c43a3bd886","slug":"7-hydroxycoumarin-glucuronide","display_name":"7-Hydroxycoumarin glucuronide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/8877866.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d\", \"start_char\": 0, \"end_char\": 2114, \"text_sha256\": \"8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme assay and HPLC metabolite identification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"7-Hydroxycoumarin plus UDP-glucuronic acid","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Bovine preparation does not identify a dominant human UGT isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Bovine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A sugar-acid donor enables the conjugation step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p8877866] Analysis of the glucuronidation of 7-hydroxycoumarin by HPLC. (1996). https://pubmed.ncbi.nlm.nih.gov/8877866/ DOI: 10.1016/0731-7085(96)01801-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver homogenate/crude UGT preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6d4e93c0-2ac4-556a-a453-a7773f625548","evidence_kind":"source_excerpt","locator":"Lines 540-551","start_line":540,"end_line":551,"excerpt":"### coumarin-ugt-conjugation\nBovine liver UGT preparation converted 7-hydroxycoumarin and UDP-glucuronic acid into its glucuronide.\nCondition category: normal\nnutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A sugar-acid donor enables the conjugation step.\norganism: Bovine\ntissue_or_cell_type: Liver homogenate/crude UGT preparation\nexperimental_model: Enzyme assay and HPLC metabolite identification\nlimitations: Bovine preparation does not identify a dominant human UGT isoform.\nexposure: 7-Hydroxycoumarin plus UDP-glucuronic acid\nevidence_span: {\"source_cache\": \"artifacts/coumarin-research/8877866.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d\", \"start_char\": 0, \"end_char\": 2114, \"text_sha256\": \"8092618f6e2773ad94670c810fb44af7c2acedc0680674872af23af8bc55e26d\"}\n[coumarin-p8877866] Analysis of the glucuronidation of 7-hydroxycoumarin by HPLC. 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