Component

Proliferation of study-specified human malignant cell lines

Proliferation of study-specified human malignant cell lines. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. The 7-hydroxycoumarin glucuronide was ineffective in the proliferation assays where parent and unconjugated metabolite were active.

    7-Hydroxycoumarin glucuronide → Proliferation of study-specified human malignant cell lines source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/9583093.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957", "start_char": 0, "end_char": 779, "text_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957"}
    experimental_model
    Comparative proliferation assays of parent, hydroxylated and glucuronidated compound
    exposure
    Parent IC50 1.59–3.57 mM; 7-hydroxy metabolite IC50 0.68–2.69 mM
    limitations
    High in vitro concentrations; effects cannot be assumed at food exposure or across cell types.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human cell lines
    plain_language
    Conjugation changed the measured biological effect.
    primary_references
    [coumarin-p9583093] Antitumor-activities of coumarin, 7-hydroxy-coumarin and its glucuronide in several human tumor cell lines. (1998). https://pubmed.ncbi.nlm.nih.gov/9583093/
    tissue_or_cell_type
    Gastric carcinoma, Caco-2, HepG2 and CCRF-CEM cells

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 462–473

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Comparative proliferation assays of parent, hydroxylated and glucuronidated compound · source_derived_draft · unverified_draft

    ### coumarin-glucuronide-null The 7-hydroxycoumarin glucuronide was ineffective in the proliferation assays where parent and unconjugated metabolite were active. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Conjugation changed the measured biological effect. organism: Human cell lines tissue_or_cell_type: Gastric carcinoma, Caco-2, HepG2 and CCRF-CEM cells experimental_model: Comparative proliferation assays of parent, hydroxylated and glucuronidated compound limitations: High in vitro concentrations; effects cannot be assumed at food exposure or across cell types. exposure: Parent IC50 1.59–3.57 mM; 7-hydroxy metabolite IC50 0.68–2.69 mM evidence_span: {"source_cache": "artifacts/coumarin-research/9583093.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957", "start_char": 0, "end_char": 779, "text_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957"} [coumarin-p9583093] Antitumor-activities of coumarin, 7-hydroxy-coumarin and its glucuronide in several human tumor cell lines. (1998). https://pubmed.ncbi.nlm.nih.gov/9583093/
    Complete structured claim and evidence

  2. 7-Hydroxycoumarin also produced dose- and time-dependent reversible growth inhibition.

    7-Hydroxycoumarin / umbelliferone → Proliferation of study-specified human malignant cell lines source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"}
    experimental_model
    Dose/time exposure and washout in malignant cell lines
    exposure
    Coumarin and 7-hydroxycoumarin in cell culture
    limitations
    Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human cell lines
    plain_language
    The metabolite had its own measured activity.
    primary_references
    [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    tissue_or_cell_type
    Study panel including HL-60, A549, LNCaP and other tumor lines

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 423–434

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Dose/time exposure and washout in malignant cell lines · source_derived_draft · unverified_draft

    ### coumarin-metabolite-cytostasis 7-Hydroxycoumarin also produced dose- and time-dependent reversible growth inhibition. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The metabolite had its own measured activity. organism: Human cell lines tissue_or_cell_type: Study panel including HL-60, A549, LNCaP and other tumor lines experimental_model: Dose/time exposure and washout in malignant cell lines limitations: Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract. exposure: Coumarin and 7-hydroxycoumarin in cell culture evidence_span: {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"} [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    Complete structured claim and evidence

  3. Coumarin inhibited proliferation with IC50 values of 1.59–3.57 mM across this four-cell-line panel.

    Coumarin → Proliferation of study-specified human malignant cell lines source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/9583093.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957", "start_char": 0, "end_char": 779, "text_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957"}
    experimental_model
    Comparative proliferation assays of parent, hydroxylated and glucuronidated compound
    exposure
    Parent IC50 1.59–3.57 mM; 7-hydroxy metabolite IC50 0.68–2.69 mM
    limitations
    High in vitro concentrations; effects cannot be assumed at food exposure or across cell types.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human cell lines
    plain_language
    This experiment required millimolar parent concentrations.
    primary_references
    [coumarin-p9583093] Antitumor-activities of coumarin, 7-hydroxy-coumarin and its glucuronide in several human tumor cell lines. (1998). https://pubmed.ncbi.nlm.nih.gov/9583093/
    tissue_or_cell_type
    Gastric carcinoma, Caco-2, HepG2 and CCRF-CEM cells

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 449–460

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Comparative proliferation assays of parent, hydroxylated and glucuronidated compound · source_derived_draft · unverified_draft

    ### coumarin-millimolar-parent Coumarin inhibited proliferation with IC50 values of 1.59–3.57 mM across this four-cell-line panel. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: This experiment required millimolar parent concentrations. organism: Human cell lines tissue_or_cell_type: Gastric carcinoma, Caco-2, HepG2 and CCRF-CEM cells experimental_model: Comparative proliferation assays of parent, hydroxylated and glucuronidated compound limitations: High in vitro concentrations; effects cannot be assumed at food exposure or across cell types. exposure: Parent IC50 1.59–3.57 mM; 7-hydroxy metabolite IC50 0.68–2.69 mM evidence_span: {"source_cache": "artifacts/coumarin-research/9583093.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957", "start_char": 0, "end_char": 779, "text_sha256": "dd691821bbdc859459d1373d8150a4579e13a0110f2d2bf1880cd895a99c5957"} [coumarin-p9583093] Antitumor-activities of coumarin, 7-hydroxy-coumarin and its glucuronide in several human tumor cell lines. (1998). https://pubmed.ncbi.nlm.nih.gov/9583093/
    Complete structured claim and evidence

  4. Coumarin inhibited proliferation reversibly after drug removal in the tested malignant cell panel.

    Coumarin → Proliferation of study-specified human malignant cell lines source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"}
    experimental_model
    Dose/time exposure and washout in malignant cell lines
    exposure
    Coumarin and 7-hydroxycoumarin in cell culture
    limitations
    Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract.
    nutrient_topic
    Coumarin research collection; topical membership is not evidence of a direct dietary effect. · Coumarin
    organism
    Human cell lines
    plain_language
    Growth slowing could reverse when exposure stopped.
    primary_references
    [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    tissue_or_cell_type
    Study panel including HL-60, A549, LNCaP and other tumor lines

    Coumarin: metabolism, signaling and nutrient connections (2026-09-17) · lines 410–421

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Dose/time exposure and washout in malignant cell lines · source_derived_draft · unverified_draft

    ### coumarin-parent-cytostasis Coumarin inhibited proliferation reversibly after drug removal in the tested malignant cell panel. Condition category: normal nutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Growth slowing could reverse when exposure stopped. organism: Human cell lines tissue_or_cell_type: Study panel including HL-60, A549, LNCaP and other tumor lines experimental_model: Dose/time exposure and washout in malignant cell lines limitations: Cell-type-specific responses; no clinical anticancer efficacy inferred; concentrations not specified in indexed abstract. exposure: Coumarin and 7-hydroxycoumarin in cell culture evidence_span: {"source_cache": "artifacts/coumarin-research/7510710.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b", "start_char": 0, "end_char": 1368, "text_sha256": "723ca14ffacb7f7130fb0ae13e07174266395023f131be84d7b6a308c38dd21b"} [coumarin-p7510710] Growth-inhibitory effects of coumarin (1,2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. (1994). https://pubmed.ncbi.nlm.nih.gov/7510710/ DOI: 10.1007/bf01377114
    Complete structured claim and evidence

In the sources

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