Component

Oxidative damage to isolated DNA in RA-metal assays

Oxidative damage to isolated DNA in RA-metal assays

6 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Bovine catalase suppressed RA/Cu(II)-induced DNA cleavage.

    Bovine catalase → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    RA/Cu(II) without this addition bovine-catalase added to RA/Cu(II) · Bovine catalase Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition 20 micromolar · Copper(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition 2 micromolar · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "bovine-catalase added to RA/Cu(II)", "comparator": "RA/Cu(II) without this addition", "endpoint": "Bovine catalase suppressed RA/Cu(II)-induced DNA cleavage.", "effect_direction": "decrease", "combination": "joint", "conditions": [{"entity_slug": "bovine-catalase", "state": "bovine-catalase added to RA/Cu(II)"}, {"entity_slug": "rosmarinic-acid", "state": "2 micromolar"}, {"entity_slug": "copper-ii", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    Bovine catalase suppressed RA/Cu(II)-induced DNA cleavage.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-catalase; Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 329–335

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. · Recorded from source · Recorded from source

    ### ra-catalase Bovine catalase suppressed RA/Cu(II)-induced DNA cleavage. Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective. Model and exposure: Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

  2. Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.

    Bathocuproine disulfonate → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    RA/Cu(II) without this addition 20 micromolar · Copper(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition 2 micromolar · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition bathocuproine-disulfonate added to RA/Cu(II) · Bathocuproine disulfonate Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "bathocuproine-disulfonate added to RA/Cu(II)", "comparator": "RA/Cu(II) without this addition", "endpoint": "Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.", "effect_direction": "decrease", "combination": "joint", "conditions": [{"entity_slug": "bathocuproine-disulfonate", "state": "bathocuproine-disulfonate added to RA/Cu(II)"}, {"entity_slug": "rosmarinic-acid", "state": "2 micromolar"}, {"entity_slug": "copper-ii", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-cu-chelator; Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 337–343

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. · Recorded from source · Recorded from source

    ### ra-cu-chelator Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage. Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective. Model and exposure: Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

  3. RA plus Cu(II) increased isolated-DNA 8-oxodG and strand/base damage.

    Rosmarinic acid → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    No RA at the same Cu(II) 20 micromolar · Copper(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    No RA at the same Cu(II) RA with Cu(II) · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "RA with Cu(II)", "comparator": "No RA at the same Cu(II)", "endpoint": "RA plus Cu(II) increased isolated-DNA 8-oxodG and strand/base damage.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "rosmarinic-acid", "state": "RA with Cu(II)"}, {"entity_slug": "copper-ii", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    RA plus Cu(II) increased isolated-DNA 8-oxodG and strand/base damage.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-cu-dna; HPLC-ECD and labeled-fragment assays; not an oral-toxicity measurement.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 305–311

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. · Recorded from source · Recorded from source

    ### ra-cu-dna RA plus Cu(II) increased isolated-DNA 8-oxodG and strand/base damage. HPLC-ECD and labeled-fragment assays; not an oral-toxicity measurement. Model and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

  4. Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.

    NADH → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    Same reaction without NADH 20 micromolar · Copper(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Same reaction without NADH 0.1–0.5 micromolar · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Same reaction without NADH 100 micromolar NADH in RA/Cu(II) reaction · NADH Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "100 micromolar NADH in RA/Cu(II) reaction", "comparator": "Same reaction without NADH", "endpoint": "Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "nadh", "state": "100 micromolar NADH in RA/Cu(II) reaction"}, {"entity_slug": "rosmarinic-acid", "state": "0.1–0.5 micromolar"}, {"entity_slug": "copper-ii", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-cu-nadh; Reductive cycling is an author-proposed explanation, not a dietary niacin interaction.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 313–319

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. · Recorded from source · Recorded from source

    ### ra-cu-nadh Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA. Reductive cycling is an author-proposed explanation, not a dietary niacin interaction. Model and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

  5. RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.

    Rosmarinic acid → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    Matched no-RA metal conditions RA with Fe(III)-EDTA, with/without NADH · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    Matched no-RA metal conditions 20 micromolar · Iron(III)-EDTA assay complex Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "RA with Fe(III)-EDTA, with/without NADH", "comparator": "Matched no-RA metal conditions", "endpoint": "RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "rosmarinic-acid", "state": "RA with Fe(III)-EDTA, with/without NADH"}, {"entity_slug": "iron-iii-edta", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-fe-null; A metal-speciation-specific null, not proof iron can never support RA oxidation.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 321–327

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. · Recorded from source · Recorded from source

    ### ra-fe-null RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase. A metal-speciation-specific null, not proof iron can never support RA oxidation. Model and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

  6. Bovine cuzn sod did not suppress RA/Cu(II)-induced DNA cleavage.

    Bovine Cu/Zn superoxide dismutase → Oxidative damage to isolated DNA in RA-metal assays Recorded from sourceungraded
    Experimental context and source evidence
    evidence_access
    Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    experimental_condition
    RA/Cu(II) without this addition 20 micromolar · Copper(II) ion Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition bovine-cuzn-sod added to RA/Cu(II) · Bovine Cu/Zn superoxide dismutase Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_condition
    RA/Cu(II) without this addition 2 micromolar · Rosmarinic acid Condition belongs to the full experimental contrast; do not separate a joint intervention.
    experimental_contrast
    {"intervention": "bovine-cuzn-sod added to RA/Cu(II)", "comparator": "RA/Cu(II) without this addition", "endpoint": "Bovine cuzn sod did not suppress RA/Cu(II)-induced DNA cleavage.", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "bovine-cuzn-sod", "state": "bovine-cuzn-sod added to RA/Cu(II)"}, {"entity_slug": "rosmarinic-acid", "state": "2 micromolar"}, {"entity_slug": "copper-ii", "state": "20 micromolar"}]} Explicit extracted experimental comparison; recorded from source.
    experimental_model
    Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.
    interpretation_status
    Recorded from source with stated access limits; ungraded, not independent replication.
    limitations
    Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.
    nutrient_topic
    Rosmarinic acid collection; exact intervention and molecular form govern interpretation. · Rosmarinic acid
    plain_language
    Bovine cuzn sod did not suppress RA/Cu(II)-induced DNA cleavage.
    primary_references
    Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7
    source_locator
    Reviewed reference ra-sod-null; Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.

    Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026) · lines 345–351

    AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed. · supports · Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. · Recorded from source · Recorded from source

    ### ra-sod-null Bovine cuzn sod did not suppress RA/Cu(II)-induced DNA cleavage. Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective. Model and exposure: Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h. Limitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation. Reference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.