{"id":"9315c0a5-7ffd-5679-8e2a-2709df17bb5c","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-cu-nadh","predicate":"increases_in_recorded_comparison","statement":"Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6139f3a4-47a7-56e5-9bd9-c43083140e9c","mechanism_event_label":"Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.","subject":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"object":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6139f3a4-47a7-56e5-9bd9-c43083140e9c","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-cu-nadh-event","event_type":"experimental_observation","label":"Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.","description":"### ra-cu-nadh\n\nAdding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.\nReductive cycling is an author-proposed explanation, not a dietary niacin interaction.\nModel and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"100 micromolar NADH in RA/Cu(II) reaction","sequence_order":0,"notes":""},{"entity":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"20 micromolar","comparator":"Same reaction without NADH","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"}},{"dimension":"experimental_condition","value_text":"100 micromolar NADH in RA/Cu(II) reaction","comparator":"Same reaction without NADH","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"0.1–0.5 micromolar","comparator":"Same reaction without NADH","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"100 micromolar NADH in RA/Cu(II) reaction\", \"comparator\": \"Same reaction without NADH\", \"endpoint\": \"Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"nadh\", \"state\": \"100 micromolar NADH in RA/Cu(II) reaction\"}, {\"entity_slug\": \"rosmarinic-acid\", \"state\": \"0.1–0.5 micromolar\"}, {\"entity_slug\": \"copper-ii\", \"state\": \"20 micromolar\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Adding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-cu-nadh; Reductive cycling is an author-proposed explanation, not a dietary niacin interaction.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6044fa49-851e-5a60-8397-129e1bb343d1","evidence_kind":"source_excerpt","locator":"Lines 313-319","start_line":313,"end_line":319,"excerpt":"### ra-cu-nadh\n\nAdding NADH enhanced low-RA/Cu(II) 8-oxodG formation by about 30-fold at 0.1–0.5 micromolar RA.\nReductive cycling is an author-proposed explanation, not a dietary niacin interaction.\nModel and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}