{"id":"d17fe998-2d03-58ce-8daa-392c31b6f80d","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-cu-chelator","predicate":"decreases_in_recorded_comparison","statement":"Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"34aa62b2-f45c-57fb-a07c-a72b0abdc50e","mechanism_event_label":"Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.","subject":{"id":"9b9bebd3-ca17-5ab1-9213-d300729516a5","slug":"bathocuproine-disulfonate","display_name":"Bathocuproine disulfonate","entity_type_key":"small_molecule"},"object":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"34aa62b2-f45c-57fb-a07c-a72b0abdc50e","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-cu-chelator-event","event_type":"experimental_observation","label":"Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.","description":"### ra-cu-chelator\n\nBathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.\nCatalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.\nModel and exposure: Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9b9bebd3-ca17-5ab1-9213-d300729516a5","slug":"bathocuproine-disulfonate","display_name":"Bathocuproine disulfonate","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"bathocuproine-disulfonate added to RA/Cu(II)","sequence_order":0,"notes":""},{"entity":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"mechanistically implicated oxidant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"162020b5-1fa5-526e-8405-452891245722","slug":"copper-i","display_name":"Copper(I) ion","entity_type_key":"ion"},"role":"chelator target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"bathocuproine-disulfonate added to RA/Cu(II)","comparator":"RA/Cu(II) without this addition","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"bathocuproine-disulfonate","display_name":"Bathocuproine disulfonate","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"20 micromolar","comparator":"RA/Cu(II) without this addition","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"}},{"dimension":"experimental_condition","value_text":"2 micromolar","comparator":"RA/Cu(II) without this addition","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"bathocuproine-disulfonate added to RA/Cu(II)\", \"comparator\": \"RA/Cu(II) without this addition\", \"endpoint\": \"Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"bathocuproine-disulfonate\", \"state\": \"bathocuproine-disulfonate added to RA/Cu(II)\"}, {\"entity_slug\": \"rosmarinic-acid\", \"state\": \"2 micromolar\"}, {\"entity_slug\": \"copper-ii\", \"state\": \"20 micromolar\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Bathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-cu-chelator; Catalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eaed19ee-bdb1-56f6-82d4-2792d2cbb3b2","evidence_kind":"source_excerpt","locator":"Lines 337-343","start_line":337,"end_line":343,"excerpt":"### ra-cu-chelator\n\nBathocuproine disulfonate suppressed RA/Cu(II)-induced DNA cleavage.\nCatalase and Cu(I)-chelator sensitivity support peroxide/Cu(I) involvement; free hydroxyl-radical scavengers were ineffective.\nModel and exposure: Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Figure 3: isolated DNA, 2 micromolar RA, 20 micromolar CuCl2; 16 h.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}