{"id":"a76b2378-1d45-5188-a777-92a5659744eb","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-fe-null","predicate":"no_detected_change_in_recorded_comparison","statement":"RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"30703ebc-3ef5-5495-8b60-be7bb62297e6","mechanism_event_label":"RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"30703ebc-3ef5-5495-8b60-be7bb62297e6","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-fe-null-event","event_type":"experimental_observation","label":"RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.","description":"### ra-fe-null\n\nRA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.\nA metal-speciation-specific null, not proof iron can never support RA oxidation.\nModel and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA with Fe(III)-EDTA, with/without NADH","sequence_order":0,"notes":""},{"entity":{"id":"8532a15a-3780-56aa-9024-0efc73f666f5","slug":"ra-cellfree-oxidative-dna-damage","display_name":"Oxidative damage to isolated DNA in RA-metal assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"20 micromolar","comparator":"Matched no-RA metal conditions","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"iron-iii-edta","display_name":"Iron(III)-EDTA assay complex","entity_type_key":"chemical_species"}},{"dimension":"experimental_condition","value_text":"RA with Fe(III)-EDTA, with/without NADH","comparator":"Matched no-RA metal conditions","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA with Fe(III)-EDTA, with/without NADH\", \"comparator\": \"Matched no-RA metal conditions\", \"endpoint\": \"RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"rosmarinic-acid\", \"state\": \"RA with Fe(III)-EDTA, with/without NADH\"}, {\"entity_slug\": \"iron-iii-edta\", \"state\": \"20 micromolar\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-fe-null; A metal-speciation-specific null, not proof iron can never support RA oxidation.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4ca5d793-4f9e-5785-a2ef-1c2a14f5338d","evidence_kind":"source_excerpt","locator":"Lines 321-327","start_line":321,"end_line":327,"excerpt":"### ra-fe-null\n\nRA with Fe(III)-EDTA produced little 8-oxodG; NADH did not produce the copper-like increase.\nA metal-speciation-specific null, not proof iron can never support RA oxidation.\nModel and exposure: Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Rosmarinic acid, a natural polyphenol, has a potential pro-oxidant risk via NADH-mediated oxidative DNA damage. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38831385/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11149181/ | https://doi.org/10.1186/s41021-024-00307-7 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Isolated DNA; 20 micromolar CuCl2 or Fe(III)-EDTA, pH 7.8, 37 °C, 16 h. No intact human cells.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}