Component

Nrf2 protein stability

Nrf2 protein stability. Species, exposure and limitations are retained in each linked claim.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Cinnamaldehyde increased NRF2 protein half-life in HCT116 cells.

    trans-Cinnamaldehyde / cinnamal → Nrf2 protein stability source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/ceylon-research/25712056.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8ad37b0dca440e7fa609a46cf02377c2979d2be8e09291a370efb963ff3c9cfd", "start_char": 0, "end_char": 1920, "text_sha256": "8ad37b0dca440e7fa609a46cf02377c2979d2be8e09291a370efb963ff3c9cfd"}
    experimental_model
    HCT116 mechanism and AOM/DSS knockout-mouse experiment
    exposure
    Purified cinnamaldehyde cell exposure and dietary supplementation in mice
    limitations
    No human cancer-prevention outcome; the abstract identifies C151 dependence but does not resolve the transfected KEAP1 construct species.
    nutrient_topic
    Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. · Ceylon cinnamon / Cinnamomum verum bark preparations
    organism
    Human HCT116 cells; KEAP1 construct species unresolved
    plain_language
    NRF2 lasted longer, allowing a stronger defense-gene response.
    primary_references
    [ceylon-p25712056] Nrf2-dependent suppression of azoxymethane/dextran sulfate sodium-induced colon carcinogenesis by the cinnamon-derived dietary factor cinnamaldehyde. (2015). https://pubmed.ncbi.nlm.nih.gov/25712056/ DOI: 10.1158/1940-6207.capr-14-0359
    tissue_or_cell_type
    Colon epithelial cancer cells and experimental inflammatory colon tumors

    Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17) · lines 610–621

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HCT116 mechanism and AOM/DSS knockout-mouse experiment · source_derived_draft · unverified_draft

    ### ceylon-nrf2-stability Cinnamaldehyde increased NRF2 protein half-life in HCT116 cells. Condition category: normal nutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. plain_language: NRF2 lasted longer, allowing a stronger defense-gene response. organism: Human HCT116 cells; KEAP1 construct species unresolved tissue_or_cell_type: Colon epithelial cancer cells and experimental inflammatory colon tumors experimental_model: HCT116 mechanism and AOM/DSS knockout-mouse experiment limitations: No human cancer-prevention outcome; the abstract identifies C151 dependence but does not resolve the transfected KEAP1 construct species. exposure: Purified cinnamaldehyde cell exposure and dietary supplementation in mice evidence_span: {"source_cache": "artifacts/ceylon-research/25712056.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8ad37b0dca440e7fa609a46cf02377c2979d2be8e09291a370efb963ff3c9cfd", "start_char": 0, "end_char": 1920, "text_sha256": "8ad37b0dca440e7fa609a46cf02377c2979d2be8e09291a370efb963ff3c9cfd"} [ceylon-p25712056] Nrf2-dependent suppression of azoxymethane/dextran sulfate sodium-induced colon carcinogenesis by the cinnamon-derived dietary factor cinnamaldehyde. (2015). https://pubmed.ncbi.nlm.nih.gov/25712056/ DOI: 10.1158/1940-6207.capr-14-0359
    Complete structured claim and evidence

  2. Sulforaphane enabled Nrf2 to escape KEAP1-dependent degradation and increased its stability.

    Sulforaphane / SFN, stereochemistry specified per study → Nrf2 protein stability source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/sulforaphane-research/14585973.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "bc2e1304879d9bd3d1b4946981f93a78d3f604c580ea252af17f645e237cadd0", "start_char": 0, "end_char": 1647, "text_sha256": "bc2e1304879d9bd3d1b4946981f93a78d3f604c580ea252af17f645e237cadd0"}
    experimental_model
    KEAP1 mutagenesis and Nrf2 stability/localization assays
    exposure
    Sulforaphane or oxidative stress; C151/C273/C288 variants
    limitations
    Individual cysteines have different roles; activating Nrf2 does not establish prevention or treatment of cancer in humans.
    nutrient_topic
    Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. · Sulforaphane / SFN, stereochemistry specified per study
    organism
    Human KEAP1 constructs in cellular experiments
    plain_language
    The cell keeps more of the regulator available.
    primary_references
    [sulforaphane-p14585973] Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of Nrf2 by chemopreventive agents and oxidative stress. (2003). https://pubmed.ncbi.nlm.nih.gov/14585973/ DOI: 10.1128/mcb.23.22.8137-8151.2003
    tissue_or_cell_type
    Ubiquitination, protein turnover and nuclear localization

    Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17) · lines 515–526

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · KEAP1 mutagenesis and Nrf2 stability/localization assays · source_derived_draft · unverified_draft

    ### sulforaphane-nrf2-stability Sulforaphane enabled Nrf2 to escape KEAP1-dependent degradation and increased its stability. Condition category: normal nutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect. plain_language: The cell keeps more of the regulator available. organism: Human KEAP1 constructs in cellular experiments tissue_or_cell_type: Ubiquitination, protein turnover and nuclear localization experimental_model: KEAP1 mutagenesis and Nrf2 stability/localization assays limitations: Individual cysteines have different roles; activating Nrf2 does not establish prevention or treatment of cancer in humans. exposure: Sulforaphane or oxidative stress; C151/C273/C288 variants evidence_span: {"source_cache": "artifacts/sulforaphane-research/14585973.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "bc2e1304879d9bd3d1b4946981f93a78d3f604c580ea252af17f645e237cadd0", "start_char": 0, "end_char": 1647, "text_sha256": "bc2e1304879d9bd3d1b4946981f93a78d3f604c580ea252af17f645e237cadd0"} [sulforaphane-p14585973] Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of Nrf2 by chemopreventive agents and oxidative stress. (2003). https://pubmed.ncbi.nlm.nih.gov/14585973/ DOI: 10.1128/mcb.23.22.8137-8151.2003
    Complete structured claim and evidence

  3. Nrf2 half-life increased from 20 to 58 minutes in the HL-60 chase experiment.

    Mangiferin → Nrf2 protein stability source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/mangiferin-research/24626801.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd", "start_char": 0, "end_char": 1659, "text_sha256": "01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd"}
    experimental_model
    Cycloheximide chase and immunoprecipitation
    exposure
    Mangiferin 50 micromolar for four hours in half-life experiment
    limitations
    Cancer cell line; no direct KEAP1-binding assignment or human systemic target-engagement inference.
    nutrient_topic
    Mangiferin research collection; topical membership is not evidence of a direct dietary effect. · Mangiferin
    organism
    Homo sapiens
    plain_language
    The protein persisted longer before degradation.
    primary_references
    [mangiferin-p24626801] Mangiferin increases Nrf2 protein stability by inhibiting its ubiquitination and degradation in human HL60 myeloid leukemia cells. (2014). https://pubmed.ncbi.nlm.nih.gov/24626801/ DOI: 10.3892/ijmm.2014.1696
    tissue_or_cell_type
    HL-60 myeloid leukemia cells

    Mangiferin: metabolism, signaling and nutrient connections (2026-09-17) · lines 523–534

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cycloheximide chase and immunoprecipitation · source_derived_draft · unverified_draft

    ### mangiferin-nrf2-half-life Nrf2 half-life increased from 20 to 58 minutes in the HL-60 chase experiment. Condition category: normal nutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The protein persisted longer before degradation. organism: Homo sapiens tissue_or_cell_type: HL-60 myeloid leukemia cells experimental_model: Cycloheximide chase and immunoprecipitation limitations: Cancer cell line; no direct KEAP1-binding assignment or human systemic target-engagement inference. exposure: Mangiferin 50 micromolar for four hours in half-life experiment evidence_span: {"source_cache": "artifacts/mangiferin-research/24626801.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd", "start_char": 0, "end_char": 1659, "text_sha256": "01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd"} [mangiferin-p24626801] Mangiferin increases Nrf2 protein stability by inhibiting its ubiquitination and degradation in human HL60 myeloid leukemia cells. (2014). https://pubmed.ncbi.nlm.nih.gov/24626801/ DOI: 10.3892/ijmm.2014.1696
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards