{"id":"55f9dc4d-4e41-5ee7-8480-6cde76fb29e9","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-nrf2-half-life","predicate":"increases","statement":"Nrf2 half-life increased from 20 to 58 minutes in the HL-60 chase experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3c0f7427-ff96-51fa-b6f9-3468c761e111","mechanism_event_label":"The protein persisted longer before degradation.","subject":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"object":{"id":"11d255d4-1166-5864-acf3-4bb5bc5de59b","slug":"nrf2-protein-stability","display_name":"Nrf2 protein stability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c0f7427-ff96-51fa-b6f9-3468c761e111","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-nrf2-half-life-event","event_type":"biochemical_relationship","label":"The protein persisted longer before degradation.","description":"Nrf2 half-life increased from 20 to 58 minutes in the HL-60 chase experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"regulated_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11d255d4-1166-5864-acf3-4bb5bc5de59b","slug":"nrf2-protein-stability","display_name":"Nrf2 protein stability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/24626801.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd\", \"start_char\": 0, \"end_char\": 1659, \"text_sha256\": \"01992b219c903dddc6e69f5fef95f73cec06cdaac0fd2c5bc2ff7b53b52192cd\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cycloheximide chase and immunoprecipitation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mangiferin 50 micromolar for four hours in half-life experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cancer cell line; no direct KEAP1-binding assignment or human systemic target-engagement inference.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The protein persisted longer before degradation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p24626801] Mangiferin increases Nrf2 protein stability by inhibiting its ubiquitination and degradation in human HL60 myeloid leukemia cells. 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