Component
Mouse nuclear NMNAT1
Context-specific entity; species, compartment and exposure are stated on each claim.
3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Nmnat1 knockdown reduced PARP1-mediated PARylation in mouse 3T3-L1 preadipocytes; catalytically active but not inactive NMNAT1 rescued the activity.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Primary full text
- experimental_model
- Mouse shRNA and enzyme re-expression experiments.
- limitations
- PARP activity is not a direct measurement of all nuclear NAD-dependent reactions.
- nutrient_topic
- NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit. · NAD+
- plain_language
- DNA-associated enzymes can depend on local NAD production.
- primary_references
- Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19) · lines 60–66
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Mouse shRNA and enzyme re-expression experiments. · source_derived_draft · unverified_draft
## nad-plus-nmnat1-loss DNA-associated enzymes can depend on local NAD production. Nmnat1 knockdown reduced PARP1-mediated PARylation in mouse 3T3-L1 preadipocytes; catalytically active but not inactive NMNAT1 rescued the activity. Model: Mouse shRNA and enzyme re-expression experiments. Limitations: PARP activity is not a direct measurement of all nuclear NAD-dependent reactions. Evidence access: Primary full text Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
Complete structured claim and evidence
What acts on it
Adipogenic induction of cytoplasmic NMNAT2 in mouse 3T3-L1 cells competed with nuclear NMNAT1 for NMN and reduced nuclear NAD+ availability.
Experimental context and source evidence
- evidence_access
- Primary full text
- experimental_model
- Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements.
- limitations
- Not evidence that human NMN supplements cause weight gain; enzyme location and differentiation state matter.
- nutrient_topic
- NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit. · NAD+
- plain_language
- Two compartments can compete for the same building material.
- primary_references
- Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19) · lines 52–58
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements. · source_derived_draft · unverified_draft
## nad-plus-nmnat2-competition Two compartments can compete for the same building material. Adipogenic induction of cytoplasmic NMNAT2 in mouse 3T3-L1 cells competed with nuclear NMNAT1 for NMN and reduced nuclear NAD+ availability. Model: Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements. Limitations: Not evidence that human NMN supplements cause weight gain; enzyme location and differentiation state matter. Evidence access: Primary full text Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
Complete structured claim and evidence
Where it participates (unsigned role)
In mouse preadipocytes, PARP1-dependent ADP-ribosylation repressed C/EBP beta adipogenic transcription; reduced nuclear NAD synthesis relieved this repression.
Experimental context and source evidence
- evidence_access
- Primary full text
- experimental_model
- Mouse 3T3-L1 transcription and differentiation experiments.
- limitations
- Cell differentiation mechanism, not a universal whole-body fat response.
- nutrient_topic
- NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit. · NAD+
- plain_language
- A local NAD change can alter a gene program through protein modification.
- primary_references
- Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19) · lines 68–74
AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Mouse 3T3-L1 transcription and differentiation experiments. · source_derived_draft · unverified_draft
## nad-plus-parp-cebpb A local NAD change can alter a gene program through protein modification. In mouse preadipocytes, PARP1-dependent ADP-ribosylation repressed C/EBP beta adipogenic transcription; reduced nuclear NAD synthesis relieved this repression. Model: Mouse 3T3-L1 transcription and differentiation experiments. Limitations: Cell differentiation mechanism, not a universal whole-body fat response. Evidence access: Primary full text Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.