{"id":"6429e29c-c76c-594a-a8f5-b5f90268bfb1","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-nmnat1-loss","predicate":"knockdown_reduces","statement":"Nmnat1 knockdown reduced PARP1-mediated PARylation in mouse 3T3-L1 preadipocytes; catalytically active but not inactive NMNAT1 rescued the activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e7bd80fd-31cd-5e4b-ad21-f0ee520a7de2","mechanism_event_label":"DNA-associated enzymes can depend on local NAD production.","subject":{"id":"ef05022f-530b-5703-8dce-c7f45abc559d","slug":"mouse-nmnat1","display_name":"Mouse nuclear NMNAT1","entity_type_key":"protein"},"object":{"id":"6a1adb55-4bdb-5638-90de-78294aafa1e4","slug":"mouse-parp1","display_name":"Mouse PARP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e7bd80fd-31cd-5e4b-ad21-f0ee520a7de2","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-nmnat1-loss-event","event_type":"observed_relationship","label":"DNA-associated enzymes can depend on local NAD production.","description":"Nmnat1 knockdown reduced PARP1-mediated PARylation in mouse 3T3-L1 preadipocytes; 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