{"id":"30a55a4a-6b16-5add-abdb-00be2cce48d5","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-nmnat2-competition","predicate":"competes_for_substrate","statement":"Adipogenic induction of cytoplasmic NMNAT2 in mouse 3T3-L1 cells competed with nuclear NMNAT1 for NMN and reduced nuclear NAD+ availability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"985c65c3-e5f8-5a60-a9cb-007a0337481a","mechanism_event_label":"Two compartments can compete for the same building material.","subject":{"id":"5d8e90b8-76b6-51ec-8256-c5018563cd3b","slug":"mouse-nmnat2","display_name":"Mouse NMNAT2","entity_type_key":"protein"},"object":{"id":"ef05022f-530b-5703-8dce-c7f45abc559d","slug":"mouse-nmnat1","display_name":"Mouse nuclear NMNAT1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"985c65c3-e5f8-5a60-a9cb-007a0337481a","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-nmnat2-competition-event","event_type":"observed_relationship","label":"Two compartments can compete for the same building material.","description":"Adipogenic induction of cytoplasmic NMNAT2 in mouse 3T3-L1 cells competed with nuclear NMNAT1 for NMN and reduced nuclear NAD+ availability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5d8e90b8-76b6-51ec-8256-c5018563cd3b","slug":"mouse-nmnat2","display_name":"Mouse NMNAT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ef05022f-530b-5703-8dce-c7f45abc559d","slug":"mouse-nmnat1","display_name":"Mouse nuclear NMNAT1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d1c59139-db61-5fa0-a25d-40c3e625152e","slug":"nmn","display_name":"NMN","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"13d04158-4c5c-5d08-b297-ceb5b73a97cf","slug":"mouse-preadipocyte-nuclear-nad","display_name":"Nuclear NAD+ in mouse preadipocytes","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that human NMN supplements cause weight gain; enzyme location and differentiation state matter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two compartments can compete for the same building material.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"473622df-223a-5b62-bb4a-670dc00fdb77","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"## nad-plus-nmnat2-competition\nTwo compartments can compete for the same building material.\nAdipogenic induction of cytoplasmic NMNAT2 in mouse 3T3-L1 cells competed with nuclear NMNAT1 for NMN and reduced nuclear NAD+ availability.\nModel: Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements.\nLimitations: Not evidence that human NMN supplements cause weight gain; enzyme location and differentiation state matter.\nEvidence access: Primary full text\nMetabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780","model_system":"Mouse preadipocyte differentiation, enzyme perturbations and compartment measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}