{"id":"83bba435-62f0-5d96-8d33-b5ede5346c9d","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-parp-cebpb","predicate":"adp_ribosylates_and_represses","statement":"In mouse preadipocytes, PARP1-dependent ADP-ribosylation repressed C/EBP beta adipogenic transcription; reduced nuclear NAD synthesis relieved this repression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b2f18858-1614-59f7-9276-9c3191783239","mechanism_event_label":"A local NAD change can alter a gene program through protein modification.","subject":{"id":"6a1adb55-4bdb-5638-90de-78294aafa1e4","slug":"mouse-parp1","display_name":"Mouse PARP1","entity_type_key":"protein"},"object":{"id":"3a6fa3b4-1baa-5181-a4b2-2a01f153a6ad","slug":"mouse-cebpb","display_name":"Mouse C/EBP beta / Cebpb","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b2f18858-1614-59f7-9276-9c3191783239","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-parp-cebpb-event","event_type":"observed_relationship","label":"A local NAD change can alter a gene program through protein modification.","description":"In mouse preadipocytes, PARP1-dependent ADP-ribosylation repressed C/EBP beta adipogenic transcription; reduced nuclear NAD synthesis relieved this repression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a1adb55-4bdb-5638-90de-78294aafa1e4","slug":"mouse-parp1","display_name":"Mouse PARP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3a6fa3b4-1baa-5181-a4b2-2a01f153a6ad","slug":"mouse-cebpb","display_name":"Mouse C/EBP beta / Cebpb","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ef05022f-530b-5703-8dce-c7f45abc559d","slug":"mouse-nmnat1","display_name":"Mouse nuclear NMNAT1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5d8e90b8-76b6-51ec-8256-c5018563cd3b","slug":"mouse-nmnat2","display_name":"Mouse NMNAT2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse 3T3-L1 transcription and differentiation experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell differentiation mechanism, not a universal whole-body fat response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A local NAD change can alter a gene program through protein modification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca20300f-f97a-5bd6-84fd-00dbfdac5e2b","evidence_kind":"source_excerpt","locator":"Lines 68-74","start_line":68,"end_line":74,"excerpt":"## nad-plus-parp-cebpb\nA local NAD change can alter a gene program through protein modification.\nIn mouse preadipocytes, PARP1-dependent ADP-ribosylation repressed C/EBP beta adipogenic transcription; reduced nuclear NAD synthesis relieved this repression.\nModel: Mouse 3T3-L1 transcription and differentiation experiments.\nLimitations: Cell differentiation mechanism, not a universal whole-body fat response.\nEvidence access: Primary full text\nMetabolic regulation of transcription through compartmentalized NAD+ biosynthesis. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29748257/ · DOI 10.1126/science.aan5780","model_system":"Mouse 3T3-L1 transcription and differentiation experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}