Component

Intestinal epithelial apical membrane

Luminal membrane domain of polarized intestinal epithelium.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Tagged human Riboflavin transporter 3 / SLC52A3 localized at the apical membrane in polarized Caco-2 and MDCK imaging experiments.

    Riboflavin transporter 3 / SLC52A3 → Intestinal epithelial apical membrane source_derived_draftungraded
    Experimental context and source evidence
    evidence_location
    Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2
    experimental_model
    Live-cell confocal imaging of tagged transporter constructs
    exposure
    Expression of fluorescent transporter constructs.
    limitations
    Tagged overexpression in epithelial models; not a universal localization map across tissues.
    nutrient_topic
    Riboflavin research collection; topical membership is not evidence of a direct dietary effect. · Riboflavin (vitamin B2)
    organism
    Human proteins in human and canine cells
    plain_language
    The riboflavin transporters occupy different parts of polarized epithelial cells.
    primary_references
    [transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. (2011). https://pubmed.ncbi.nlm.nih.gov/21854757/ DOI: 10.1016/j.bbamem.2011.08.004
    tissue_or_cell_type
    Polarized Caco-2 and MDCK cells

    Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 202–213

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Live-cell confocal imaging of tagged transporter constructs · source_derived_draft · unverified_draft

    ### transport-slc52a3-localization Tagged human Riboflavin transporter 3 / SLC52A3 localized at the apical membrane in polarized Caco-2 and MDCK imaging experiments. Condition category: normal nutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The riboflavin transporters occupy different parts of polarized epithelial cells. organism: Human proteins in human and canine cells tissue_or_cell_type: Polarized Caco-2 and MDCK cells experimental_model: Live-cell confocal imaging of tagged transporter constructs limitations: Tagged overexpression in epithelial models; not a universal localization map across tissues. exposure: Expression of fluorescent transporter constructs. evidence_location: Live-cell imaging; historical RFT1/2/3 correspond to RFVT1/3/2 [transport-localization-2011] Differential expression of human riboflavin transporters -1, -2, and -3 in polarized epithelia: a key role for hRFT-2 in intestinal riboflavin uptake. (2011). https://pubmed.ncbi.nlm.nih.gov/21854757/ DOI: 10.1016/j.bbamem.2011.08.004
    Complete structured claim and evidence

  2. NIS immunolocalization in rat small-intestinal enterocytes was restricted to their apical surface.

    Rat sodium/iodide symporter / Slc5a5 → Intestinal epithelial apical membrane source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    Rat and mouse intestinal localization, intestinal brush-border vesicles and IEC-6 cells
    exposure
    Tissue localization; no intake intervention used for this claim.
    limitations
    Anatomical localization does not quantify the fraction of human iodine absorbed through this route.
    nutrient_topic
    Iodine research collection; topical membership is not evidence of a direct dietary effect. · Iodine
    organism
    Rattus norvegicus
    plain_language
    In the intestine, NIS faces the dietary contents.
    primary_references
    [iodine-trans-intestine2009] The Na+/I- symporter mediates active iodide uptake in the intestine. (2009). https://pubmed.ncbi.nlm.nih.gov/19052257/ DOI: 10.1152/ajpcell.00509.2008
    tissue_or_cell_type
    Small-intestinal enterocytes

    Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17) · lines 245–256

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat and mouse intestinal localization, intestinal brush-border vesicles and IEC-6 cells · source_derived_draft · unverified_draft

    ### iodine-trans-intestinal-location NIS immunolocalization in rat small-intestinal enterocytes was restricted to their apical surface. Condition category: normal nutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect. plain_language: In the intestine, NIS faces the dietary contents. organism: Rattus norvegicus tissue_or_cell_type: Small-intestinal enterocytes experimental_model: Rat and mouse intestinal localization, intestinal brush-border vesicles and IEC-6 cells limitations: Anatomical localization does not quantify the fraction of human iodine absorbed through this route. exposure: Tissue localization; no intake intervention used for this claim. cross_nutrient: false [iodine-trans-intestine2009] The Na+/I- symporter mediates active iodide uptake in the intestine. (2009). https://pubmed.ncbi.nlm.nih.gov/19052257/ DOI: 10.1152/ajpcell.00509.2008
    Complete structured claim and evidence

  3. Functional full-length human SMVT-GFP localized to the apical membrane in the studied polarized intestinal epithelial cell lines.

    Human sodium-dependent multivitamin transporter / SLC5A6 → Intestinal epithelial apical membrane source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    true
    experimental_model
    Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines
    exposure
    Full-length human SMVT-GFP expression and polarized membrane imaging.
    limitations
    Abstract-only; fluorescent fusion-protein localization in cell lines is not a measurement of net intestinal pantothenate absorption. The abstract does not name the intestinal cell line.
    nutrient_topic
    Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. · Pantothenate (vitamin B5)
    organism
    Homo sapiens (transporter); expression-host species not specified in the retrieved abstract
    plain_language
    In the intestinal cell models, SMVT sits on the surface facing the intestinal contents.
    primary_references
    [b5-trans-targeting2009] Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19211916/ DOI: 10.1152/ajpcell.00396.2008
    tissue_or_cell_type
    Polarized intestinal epithelial cell model

    Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17) · lines 223–234

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines · source_derived_draft · unverified_draft

    ### b5-trans-epithelial-apical-targeting Functional full-length human SMVT-GFP localized to the apical membrane in the studied polarized intestinal epithelial cell lines. Condition category: normal nutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. plain_language: In the intestinal cell models, SMVT sits on the surface facing the intestinal contents. organism: Homo sapiens (transporter); expression-host species not specified in the retrieved abstract tissue_or_cell_type: Polarized intestinal epithelial cell model experimental_model: Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines limitations: Abstract-only; fluorescent fusion-protein localization in cell lines is not a measurement of net intestinal pantothenate absorption. The abstract does not name the intestinal cell line. exposure: Full-length human SMVT-GFP expression and polarized membrane imaging. cross_nutrient: true [b5-trans-targeting2009] Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19211916/ DOI: 10.1152/ajpcell.00396.2008
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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