{"id":"94a167e3-2ae6-594a-bc94-1106efc6b805","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-epithelial-apical-targeting","predicate":"localizes_to","statement":"Functional full-length human SMVT-GFP localized to the apical membrane in the studied polarized intestinal epithelial cell lines.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"55a71f8a-fbb8-5968-ae5c-41eb871786ec","mechanism_event_label":"In the intestinal cell models, SMVT sits on the surface facing the intestinal contents.","subject":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"object":{"id":"70ddec47-5cc3-5b1c-9d17-c0c7f1609d49","slug":"intestinal-epithelial-apical-membrane","display_name":"Intestinal epithelial apical membrane","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"55a71f8a-fbb8-5968-ae5c-41eb871786ec","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-epithelial-apical-targeting-event","event_type":"biochemical_relationship","label":"In the intestinal cell models, SMVT sits on the surface facing the intestinal contents.","description":"Functional full-length human SMVT-GFP localized to the apical membrane in the studied polarized intestinal epithelial cell lines.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"shared_transporter_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":"Pantothenate transport is established independently; this experiment measured localization and biotin uptake."},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"functional_assay_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"70ddec47-5cc3-5b1c-9d17-c0c7f1609d49","slug":"intestinal-epithelial-apical-membrane","display_name":"Intestinal epithelial apical membrane","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Full-length human SMVT-GFP expression and polarized membrane imaging.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-only; fluorescent fusion-protein localization in cell lines is not a measurement of net intestinal pantothenate absorption. The abstract does not name the intestinal cell line.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens (transporter); expression-host species not specified in the retrieved abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In the intestinal cell models, SMVT sits on the surface facing the intestinal contents.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-targeting2009] Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19211916/ DOI: 10.1152/ajpcell.00396.2008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized intestinal epithelial cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2c551a17-0629-529a-8625-62bc0301493a","evidence_kind":"source_excerpt","locator":"Lines 223-234","start_line":223,"end_line":234,"excerpt":"### b5-trans-epithelial-apical-targeting\nFunctional full-length human SMVT-GFP localized to the apical membrane in the studied polarized intestinal epithelial cell lines.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In the intestinal cell models, SMVT sits on the surface facing the intestinal contents.\norganism: Homo sapiens (transporter); expression-host species not specified in the retrieved abstract\ntissue_or_cell_type: Polarized intestinal epithelial cell model\nexperimental_model: Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines\nlimitations: Abstract-only; fluorescent fusion-protein localization in cell lines is not a measurement of net intestinal pantothenate absorption. The abstract does not name the intestinal cell line.\nexposure: Full-length human SMVT-GFP expression and polarized membrane imaging.\ncross_nutrient: true\n[b5-trans-targeting2009] Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19211916/ DOI: 10.1152/ajpcell.00396.2008","model_system":"Functional human SMVT-GFP expression and imaging in polarized renal and intestinal epithelial cell lines","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-targeting2009] Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. 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