Component
ABCB7 knockdown in human cells
3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
ABCB7 knockdown did not similarly impair the tested CCCP-induced ISR.
Experimental context and source evidence
- access_level
- selected_indexed_full_text_passages
- dose
- Unresolved; not inferred from another panel
- duration
- 72-hour siRNA; CCCP during final 16 hours
- endpoint
- ATF4/ISR immunoblot readout
- evidence_cache
- artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21
- experimental_model
- HeLa
- exposure
- CCCP concentration unresolved
- limitations
- Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
- organism
- Human
- primary_locator
- https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J
- primary_references
- https://doi.org/10.1016/j.molcel.2023.05.031
Requirements for iron-triggered DELE1 signaling · lines 34–45
Primary study 10.1016/j.molcel.2023.05.031; targeted counterevidence curation, 2026-09-20. · supports · HeLa · source_derived_draft · unverified_draft
ABCB7 knockdown did not similarly impair the tested CCCP-induced ISR. primary_references: https://doi.org/10.1016/j.molcel.2023.05.031 primary_locator: https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J evidence_cache: artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21 access_level: selected_indexed_full_text_passages organism: Human experimental_model: HeLa duration: 72-hour siRNA; CCCP during final 16 hours exposure: CCCP concentration unresolved dose: Unresolved; not inferred from another panel endpoint: ATF4/ISR immunoblot readout limitations: Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
Complete structured claim and evidenceABCB7 knockdown attenuated iron-chelator-induced ATF4 expression.
Experimental context and source evidence
- access_level
- selected_indexed_full_text_passages
- dose
- Unresolved; not inferred from another panel
- duration
- 72-hour siRNA; chelator during final 16 hours
- endpoint
- ATF4/ISR immunoblot readout
- evidence_cache
- artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21
- experimental_model
- HeLa
- exposure
- DFO or DFP; concentrations unresolved
- limitations
- Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
- organism
- Human
- primary_locator
- https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J
- primary_references
- https://doi.org/10.1016/j.molcel.2023.05.031
Requirements for iron-triggered DELE1 signaling · lines 6–17
Primary study 10.1016/j.molcel.2023.05.031; targeted counterevidence curation, 2026-09-20. · supports · HeLa · source_derived_draft · unverified_draft
ABCB7 knockdown attenuated iron-chelator-induced ATF4 expression. primary_references: https://doi.org/10.1016/j.molcel.2023.05.031 primary_locator: https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J evidence_cache: artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21 access_level: selected_indexed_full_text_passages organism: Human experimental_model: HeLa duration: 72-hour siRNA; chelator during final 16 hours exposure: DFO or DFP; concentrations unresolved dose: Unresolved; not inferred from another panel endpoint: ATF4/ISR immunoblot readout limitations: Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
Complete structured claim and evidenceABCB7 knockdown reduced DELE1 stabilization during iron chelation.
Experimental context and source evidence
- access_level
- selected_indexed_full_text_passages
- dose
- Unresolved; not inferred from another panel
- duration
- 72-hour siRNA; DFO for 16 hours; CHX chase final 30 minutes
- endpoint
- DELE1 protein stability immunoblot readout
- evidence_cache
- artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21
- experimental_model
- Endogenous DELE1-HA HEK293T
- exposure
- DFO and CHX concentrations unresolved
- limitations
- Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
- organism
- Human
- primary_locator
- https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6K/L
- primary_references
- https://doi.org/10.1016/j.molcel.2023.05.031
Requirements for iron-triggered DELE1 signaling · lines 20–31
Primary study 10.1016/j.molcel.2023.05.031; targeted counterevidence curation, 2026-09-20. · supports · Endogenous DELE1-HA HEK293T · source_derived_draft · unverified_draft
ABCB7 knockdown reduced DELE1 stabilization during iron chelation. primary_references: https://doi.org/10.1016/j.molcel.2023.05.031 primary_locator: https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6K/L evidence_cache: artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21 access_level: selected_indexed_full_text_passages organism: Human experimental_model: Endogenous DELE1-HA HEK293T duration: 72-hour siRNA; DFO for 16 hours; CHX chase final 30 minutes exposure: DFO and CHX concentrations unresolved dose: Unresolved; not inferred from another panel endpoint: DELE1 protein stability immunoblot readout limitations: Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.