{"id":"1b2a64b3-d5c9-53de-8505-683d5177264f","stable_key":"0a78b350-a082-585a-984b-3a9f329e1574:abcb7-loss-cccp-isr","predicate":"has_contextual_experimental_effect","statement":"ABCB7 knockdown did not similarly impair the tested CCCP-induced ISR.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"20765825-905e-5ca6-ab35-8313e0b18587","mechanism_event_label":"abcb7 loss cccp isr","subject":{"id":"a532c9f7-60f9-5075-93d5-ba7f9d611a38","slug":"human-abcb7-knockdown","display_name":"ABCB7 knockdown in human cells","entity_type_key":"protein_state"},"object":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"20765825-905e-5ca6-ab35-8313e0b18587","stable_key":"0a78b350-a082-585a-984b-3a9f329e1574:abcb7-loss-cccp-isr","event_type":"experimental_result","label":"abcb7 loss cccp isr","description":"ABCB7 knockdown did not similarly impair the tested CCCP-induced ISR.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a532c9f7-60f9-5075-93d5-ba7f9d611a38","slug":"human-abcb7-knockdown","display_name":"ABCB7 knockdown in human cells","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a9dd2b3-2552-5387-a086-66bea9f56058","slug":"atf4","display_name":"Human activating transcription factor 4 / ATF4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"selected_indexed_full_text_passages","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Unresolved; not inferred from another panel","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"72-hour siRNA; CCCP during final 16 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"ATF4/ISR immunoblot readout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HeLa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"CCCP concentration unresolved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1016/j.molcel.2023.05.031","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"96ecc0b1-82f5-5e9d-be89-77037de485d6","evidence_kind":"source_excerpt","locator":"Lines 34-45","start_line":34,"end_line":45,"excerpt":"ABCB7 knockdown did not similarly impair the tested CCCP-induced ISR.\nprimary_references: https://doi.org/10.1016/j.molcel.2023.05.031\nprimary_locator: https://pmc.ncbi.nlm.nih.gov/articles/PMC10329284/ Figure 6I/J\nevidence_cache: artifacts/discovery-research/round2-sources/iscu-counterevidence-search.json; SHA256 91e6dd454b1fdecf0cf4d29d2579bf1eba148109e446059f91ea966f8bf0de21\naccess_level: selected_indexed_full_text_passages\norganism: Human\nexperimental_model: HeLa\nduration: 72-hour siRNA; CCCP during final 16 hours\nexposure: CCCP concentration unresolved\ndose: Unresolved; not inferred from another panel\nendpoint: ATF4/ISR immunoblot readout\nlimitations: Selected primary Results and figure legends accessed through indexed text. Supplements were not independently inspected. These results constrain a hypothesis; they do not test SLC25A39 loss or establish its bypass. Exact iron sensor and transporter substrate remain unresolved.","model_system":"HeLa","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Authored paraphrase with primary locator and unresolved conditions.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"eeffd0c6-7b5e-5ec2-944b-6db3981fb281","stable_key":"import-0a78b350-a082-585a-984b-3a9f329e1574","title":"Requirements for iron-triggered DELE1 signaling","document_type":"imported_text","citation_label":"Primary study 10.1016/j.molcel.2023.05.031; targeted counterevidence curation, 2026-09-20.","file_path":"","sha256":"a51546326ca2ae4bbae2be44808672a526607fc7b68ade25f143a7a93cc08cf0","revision_id":"0d367584-8f47-5eda-946d-3d9f780b9b74","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}