Component

Cellular dehydroascorbic acid uptake

Cellular dehydroascorbic acid uptake

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. D-glucose inhibited GLUT1- and GLUT3-mediated DHA uptake in the oocyte expression system.

    D-glucose → Cellular dehydroascorbic acid uptake source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    true
    experimental_model
    Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression
    exposure
    D-glucose during heterologous-transporter DHA uptake
    limitations
    Not evidence that eating carbohydrate causes vitamin C deficiency; do not generalize across transporters, redox forms or tissue kinetics.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Xenopus laevis host; mammalian clones
    plain_language
    Glucose competed with oxidized vitamin C uptake in this laboratory transport model.
    primary_references
    [rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982
    tissue_or_cell_type
    Oocyte membrane

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 273–284

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression · source_derived_draft · unverified_draft

    ### vc-transport-glut-glucose-inhibition D-glucose inhibited GLUT1- and GLUT3-mediated DHA uptake in the oocyte expression system. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: Glucose competed with oxidized vitamin C uptake in this laboratory transport model. organism: Xenopus laevis host; mammalian clones tissue_or_cell_type: Oocyte membrane experimental_model: Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression limitations: Not evidence that eating carbohydrate causes vitamin C deficiency; do not generalize across transporters, redox forms or tissue kinetics. exposure: D-glucose during heterologous-transporter DHA uptake cross_nutrient: true [rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982
    Complete structured claim and evidence

  2. Preincubation of isolated rat adipocytes with 0.67 µM insulin increased DHA transport 6–8-fold; intracellular DHA reduction was complete both before and after insulin.

    Insulin → Cellular dehydroascorbic acid uptake source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    true
    experimental_model
    Isolated rat adipocytes and GLUT4-expressing Xenopus oocytes
    exposure
    0.67 µM insulin preincubation
    limitations
    Pharmacological cell exposure; no claim about human vitamin C requirements in diabetes.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Rattus norvegicus
    plain_language
    Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction.
    primary_references
    [rumsey2000] Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes. (2000). https://pubmed.ncbi.nlm.nih.gov/10862609/ DOI: 10.1074/jbc.m000988200
    tissue_or_cell_type
    Adipocytes

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 299–310

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Isolated rat adipocytes and GLUT4-expressing Xenopus oocytes · source_derived_draft · unverified_draft

    ### vc-transport-insulin-dha-uptake Preincubation of isolated rat adipocytes with 0.67 µM insulin increased DHA transport 6–8-fold; intracellular DHA reduction was complete both before and after insulin. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction. organism: Rattus norvegicus tissue_or_cell_type: Adipocytes experimental_model: Isolated rat adipocytes and GLUT4-expressing Xenopus oocytes limitations: Pharmacological cell exposure; no claim about human vitamin C requirements in diabetes. exposure: 0.67 µM insulin preincubation cross_nutrient: true [rumsey2000] Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes. (2000). https://pubmed.ncbi.nlm.nih.gov/10862609/ DOI: 10.1074/jbc.m000988200
    Complete structured claim and evidence

  3. Montel-Hagen et al. reported that 5 mM glucose did not inhibit DHA accumulation in mature human erythrocytes and interpreted this as preferential DHA transport.

    D-glucose → Cellular dehydroascorbic acid uptake source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    true
    experimental_model
    Human erythroid progenitors, mature RBCs and A431 stomatin transfection
    exposure
    5 mM glucose with radiolabeled DHA; room-temperature uptake time courses
    limitations
    Published interpretation challenged by kinetic analyses that distinguish transport, intracellular reduction and sugar loading.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Homo sapiens
    plain_language
    One study reported that glucose did not compete with oxidized vitamin C uptake in mature red cells.
    primary_references
    [montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042
    tissue_or_cell_type
    Mature erythrocytes

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 520–531

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human erythroid progenitors, mature RBCs and A431 stomatin transfection · source_derived_draft · unverified_draft

    ### vc-transport-rbc-glucose-noncompetition Montel-Hagen et al. reported that 5 mM glucose did not inhibit DHA accumulation in mature human erythrocytes and interpreted this as preferential DHA transport. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: One study reported that glucose did not compete with oxidized vitamin C uptake in mature red cells. organism: Homo sapiens tissue_or_cell_type: Mature erythrocytes experimental_model: Human erythroid progenitors, mature RBCs and A431 stomatin transfection limitations: Published interpretation challenged by kinetic analyses that distinguish transport, intracellular reduction and sugar loading. exposure: 5 mM glucose with radiolabeled DHA; room-temperature uptake time courses cross_nutrient: true [montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042
    Complete structured claim and evidence

  4. Stomatin transfection increased DHA uptake in human A431 cells while GLUT1 abundance was unchanged; GLUT1 knockdown reduced uptake.

    Human stomatin / STOM → Cellular dehydroascorbic acid uptake source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    Human erythroid progenitors, mature RBCs and A431 stomatin transfection
    exposure
    Stomatin transfection; 30- and 300-second uptake assays
    limitations
    Cancer cell line; preferential-transport interpretation is disputed by later erythrocyte kinetics.
    nutrient_topic
    Vitamin C research collection; topical membership is not evidence of a direct dietary effect. · Vitamin C
    organism
    Homo sapiens
    plain_language
    Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein.
    primary_references
    [montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042
    tissue_or_cell_type
    A431 cells

    Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 507–518

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human erythroid progenitors, mature RBCs and A431 stomatin transfection · source_derived_draft · unverified_draft

    ### vc-transport-stomatin-dha Stomatin transfection increased DHA uptake in human A431 cells while GLUT1 abundance was unchanged; GLUT1 knockdown reduced uptake. Condition category: normal nutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect. plain_language: Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein. organism: Homo sapiens tissue_or_cell_type: A431 cells experimental_model: Human erythroid progenitors, mature RBCs and A431 stomatin transfection limitations: Cancer cell line; preferential-transport interpretation is disputed by later erythrocyte kinetics. exposure: Stomatin transfection; 30- and 300-second uptake assays cross_nutrient: false [montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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