{"id":"c3d2686f-225e-552b-9b2f-fa2c2979934c","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-stomatin-dha","predicate":"overexpression-increases","statement":"Stomatin transfection increased DHA uptake in human A431 cells while GLUT1 abundance was unchanged; GLUT1 knockdown reduced uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ffe581c5-bba9-563e-8a6c-2e3eba484524","mechanism_event_label":"Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein.","subject":{"id":"160a281d-f2f1-5edd-90b5-32aeaa70f2ba","slug":"stom","display_name":"Human stomatin / STOM","entity_type_key":"protein"},"object":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ffe581c5-bba9-563e-8a6c-2e3eba484524","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-stomatin-dha-event","event_type":"biochemical_relationship","label":"Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein.","description":"Stomatin transfection increased DHA uptake in human A431 cells while GLUT1 abundance was unchanged; GLUT1 knockdown reduced uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ffb5e632-9a8e-5bed-97eb-56a9ecdd856f","slug":"slc2a1","display_name":"Human glucose transporter 1 / SLC2A1","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"160a281d-f2f1-5edd-90b5-32aeaa70f2ba","slug":"stom","display_name":"Human stomatin / STOM","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human erythroid progenitors, mature RBCs and A431 stomatin transfection","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Stomatin transfection; 30- and 300-second uptake assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cancer cell line; preferential-transport interpretation is disputed by later erythrocyte kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"A431 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"38690019-1059-5d45-abe7-f4329c01a5b7","evidence_kind":"source_excerpt","locator":"Lines 507-518","start_line":507,"end_line":518,"excerpt":"### vc-transport-stomatin-dha\nStomatin transfection increased DHA uptake in human A431 cells while GLUT1 abundance was unchanged; GLUT1 knockdown reduced uptake.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Stomatin changed oxidized vitamin C uptake without simply adding more GLUT1 protein.\norganism: Homo sapiens\ntissue_or_cell_type: A431 cells\nexperimental_model: Human erythroid progenitors, mature RBCs and A431 stomatin transfection\nlimitations: Cancer cell line; preferential-transport interpretation is disputed by later erythrocyte kinetics.\nexposure: Stomatin transfection; 30- and 300-second uptake assays\ncross_nutrient: false\n[montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042","model_system":"Human erythroid progenitors, mature RBCs and A431 stomatin transfection","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [montelhagen2008] Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. (2008). https://pubmed.ncbi.nlm.nih.gov/18358815/ DOI: 10.1016/j.cell.2008.01.042","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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