{"id":"98dd2373-3a08-5de1-85e1-c4e095c7a958","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-insulin-dha-uptake","predicate":"increases","statement":"Preincubation of isolated rat adipocytes with 0.67 µM insulin increased DHA transport 6–8-fold; intracellular DHA reduction was complete both before and after insulin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f65e45bb-dd79-5ca7-9f15-41936688b898","mechanism_event_label":"Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction.","subject":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"object":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f65e45bb-dd79-5ca7-9f15-41936688b898","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-insulin-dha-uptake-event","event_type":"biochemical_relationship","label":"Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction.","description":"Preincubation of isolated rat adipocytes with 0.67 µM insulin increased DHA transport 6–8-fold; intracellular DHA reduction was complete both before and after insulin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b355feba-1bd3-55d6-9f12-54b9fbadfd58","slug":"rat-slc2a4","display_name":"Rat glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat adipocytes and GLUT4-expressing Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"0.67 µM insulin preincubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological cell exposure; no claim about human vitamin C requirements in diabetes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rumsey2000] Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes. (2000). https://pubmed.ncbi.nlm.nih.gov/10862609/ DOI: 10.1074/jbc.m000988200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1502d719-6f15-5e42-8a8c-5314c58cfb49","evidence_kind":"source_excerpt","locator":"Lines 299-310","start_line":299,"end_line":310,"excerpt":"### vc-transport-insulin-dha-uptake\nPreincubation of isolated rat adipocytes with 0.67 µM insulin increased DHA transport 6–8-fold; intracellular DHA reduction was complete both before and after insulin.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Insulin increased oxidized vitamin C entry into rat fat cells, rather than simply speeding its internal reduction.\norganism: Rattus norvegicus\ntissue_or_cell_type: Adipocytes\nexperimental_model: Isolated rat adipocytes and GLUT4-expressing Xenopus oocytes\nlimitations: Pharmacological cell exposure; no claim about human vitamin C requirements in diabetes.\nexposure: 0.67 µM insulin preincubation\ncross_nutrient: true\n[rumsey2000] Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes. 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