{"id":"c0d34ce5-f442-56c7-9b52-975c849e0d89","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-glut-glucose-inhibition","predicate":"inhibits","statement":"D-glucose inhibited GLUT1- and GLUT3-mediated DHA uptake in the oocyte expression system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"18be7899-d4b7-5c33-837e-9363ad9e1a76","mechanism_event_label":"Glucose competed with oxidized vitamin C uptake in this laboratory transport model.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"18be7899-d4b7-5c33-837e-9363ad9e1a76","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-glut-glucose-inhibition-event","event_type":"biochemical_relationship","label":"Glucose competed with oxidized vitamin C uptake in this laboratory transport model.","description":"D-glucose inhibited GLUT1- and GLUT3-mediated DHA uptake in the oocyte expression system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d4b7f53a-733f-5794-ab85-8069d264cacb","slug":"mammalian-glut1-orthologs","display_name":"Mammalian GLUT1 orthologs","entity_type_key":"protein_family"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a8b00caa-810d-5cd5-94d7-13d9ad34c8a0","slug":"mammalian-glut3-orthologs","display_name":"Mammalian GLUT3 orthologs","entity_type_key":"protein_family"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"57c3c5fb-5ee3-5af6-83f0-c8b32387e749","slug":"dha-cellular-uptake","display_name":"Cellular dehydroascorbic acid uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"D-glucose during heterologous-transporter DHA uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that eating carbohydrate causes vitamin C deficiency; do not generalize across transporters, redox forms or tissue kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Xenopus laevis host; mammalian clones","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glucose competed with oxidized vitamin C uptake in this laboratory transport model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3a9ec4e4-1478-53ef-8ade-3cfe392b023e","evidence_kind":"source_excerpt","locator":"Lines 273-284","start_line":273,"end_line":284,"excerpt":"### vc-transport-glut-glucose-inhibition\nD-glucose inhibited GLUT1- and GLUT3-mediated DHA uptake in the oocyte expression system.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glucose competed with oxidized vitamin C uptake in this laboratory transport model.\norganism: Xenopus laevis host; mammalian clones\ntissue_or_cell_type: Oocyte membrane\nexperimental_model: Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression\nlimitations: Not evidence that eating carbohydrate causes vitamin C deficiency; do not generalize across transporters, redox forms or tissue kinetics.\nexposure: D-glucose during heterologous-transporter DHA uptake\ncross_nutrient: true\n[rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982","model_system":"Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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