Component
Müller-directed DES1 loss
Pdgfra-Cre-mediated excision of Degs1 exon 2 in the mouse; residual whole-retina DES1 activity is expected from other cell types.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Müller-directed Degs1 deletion did not slow cone dark adaptation after greater than 90% pigment bleaching in isolated mouse retinas or intact eyes.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Figure 7C-D
- experimental_model
- Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background
- exposure
- 505-nm ex vivo or 520-nm in vivo bleach; mutant versus heterozygous littermate controls.
- limitations
- Does not exclude other DES1 functions, residual extra-Müller activity, or species-specific contributions.
- nutrient
- Vitamin A · Vitamin A
- nutrient_topic
- Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
- organism
- Mus musculus
- plain_language
- The tested mice regenerated cone pigment normally despite targeted DES1 loss.
- primary_references
- [vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r
- tissue_or_cell_type
- neural retina and intact-eye recordings
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 618–631
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background · source_derived_draft · unverified_draft
### vae-des1-deletion-cone-recovery Müller-directed Degs1 deletion did not slow cone dark adaptation after greater than 90% pigment bleaching in isolated mouse retinas or intact eyes. Condition category: machinery_impairment nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: The tested mice regenerated cone pigment normally despite targeted DES1 loss. organism: Mus musculus tissue_or_cell_type: neural retina and intact-eye recordings experimental_model: Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background limitations: Does not exclude other DES1 functions, residual extra-Müller activity, or species-specific contributions. exposure: 505-nm ex vivo or 520-nm in vivo bleach; mutant versus heterozygous littermate controls. cross_nutrient: false evidence_location: Figure 7C-D nutrient: Vitamin A [vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r
Complete structured claim and evidenceMüller-directed Degs1 conditional mutants showed slightly faster cone photoresponse termination than controls in ex vivo retinal recordings.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Figure 7A-B; Discussion
- experimental_model
- Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background
- exposure
- Dark-adapted transretinal cone flash responses.
- limitations
- The responsible cellular or lipid mechanism was unresolved; do not equate termination kinetics with pigment regeneration.
- nutrient
- Vitamin A · Vitamin A
- nutrient_topic
- Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
- organism
- Mus musculus
- plain_language
- The response switched off faster even though pigment recovery was preserved.
- primary_references
- [vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r
- tissue_or_cell_type
- isolated retina
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 633–646
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background · source_derived_draft · unverified_draft
### vae-des1-deletion-cone-termination Müller-directed Degs1 conditional mutants showed slightly faster cone photoresponse termination than controls in ex vivo retinal recordings. Condition category: machinery_impairment nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: The response switched off faster even though pigment recovery was preserved. organism: Mus musculus tissue_or_cell_type: isolated retina experimental_model: Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background limitations: The responsible cellular or lipid mechanism was unresolved; do not equate termination kinetics with pigment regeneration. exposure: Dark-adapted transretinal cone flash responses. cross_nutrient: false evidence_location: Figure 7A-B; Discussion nutrient: Vitamin A [vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.