{"id":"87e8ed91-a5e9-5ec2-819d-7aec84caf726","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-des1-deletion-cone-termination","predicate":"accelerates","statement":"Müller-directed Degs1 conditional mutants showed slightly faster cone photoresponse termination than controls in ex vivo retinal recordings.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"bf42769a-85fc-50b7-84ee-ea534f22b64d","mechanism_event_label":"The response switched off faster even though pigment recovery was preserved.","subject":{"id":"0af2b309-dae6-5e4d-9d2c-4ac1faae585a","slug":"degs1-muller-loss","display_name":"Müller-directed DES1 loss","entity_type_key":"protein_state"},"object":{"id":"c0ab6de2-300e-58e2-9f82-ed5243c1b213","slug":"cone-photoresponse-termination","display_name":"Cone photoresponse termination","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bf42769a-85fc-50b7-84ee-ea534f22b64d","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-des1-deletion-cone-termination-event","event_type":"observed_intervention","label":"The response switched off faster even though pigment recovery was preserved.","description":"Müller-directed Degs1 conditional mutants showed slightly faster cone photoresponse termination than controls in ex vivo retinal recordings.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0af2b309-dae6-5e4d-9d2c-4ac1faae585a","slug":"degs1-muller-loss","display_name":"Müller-directed DES1 loss","entity_type_key":"protein_state"},"role":"conditional perturbation","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"743b0a10-1977-5b02-a116-1c53c6700b3d","slug":"degs1","display_name":"Dihydroceramide desaturase 1 / DES1","entity_type_key":"protein"},"role":"targeted protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c0ab6de2-300e-58e2-9f82-ed5243c1b213","slug":"cone-photoresponse-termination","display_name":"Cone photoresponse termination","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 7A-B; Discussion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dark-adapted transretinal cone flash responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The responsible cellular or lipid mechanism was unresolved; do not equate termination kinetics with pigment regeneration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The response switched off faster even though pigment recovery was preserved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"isolated retina","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"a817612f-44e1-566e-b583-3a9aa6d5d65a","evidence_kind":"source_excerpt","locator":"Lines 633-646","start_line":633,"end_line":646,"excerpt":"### vae-des1-deletion-cone-termination\nMüller-directed Degs1 conditional mutants showed slightly faster cone photoresponse termination than controls in ex vivo retinal recordings.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The response switched off faster even though pigment recovery was preserved.\norganism: Mus musculus\ntissue_or_cell_type: isolated retina\nexperimental_model: Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background\nlimitations: The responsible cellular or lipid mechanism was unresolved; do not equate termination kinetics with pigment regeneration.\nexposure: Dark-adapted transretinal cone flash responses.\ncross_nutrient: false\nevidence_location: Figure 7A-B; Discussion\nnutrient: Vitamin A\n[vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. 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