{"id":"989350a5-7aaa-50dc-ab71-5a6231bbe5fe","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-des1-deletion-cone-recovery","predicate":"does_not_slow_measured","statement":"Müller-directed Degs1 deletion did not slow cone dark adaptation after greater than 90% pigment bleaching in isolated mouse retinas or intact eyes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ada9fd3c-3cd0-5c16-a9d2-90246f2445c4","mechanism_event_label":"The tested mice regenerated cone pigment normally despite targeted DES1 loss.","subject":{"id":"0af2b309-dae6-5e4d-9d2c-4ac1faae585a","slug":"degs1-muller-loss","display_name":"Müller-directed DES1 loss","entity_type_key":"protein_state"},"object":{"id":"a4a3ca81-92c9-5e98-b325-7347e1a28b3e","slug":"cone-dark-adaptation","display_name":"Cone dark adaptation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ada9fd3c-3cd0-5c16-a9d2-90246f2445c4","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-des1-deletion-cone-recovery-event","event_type":"observed_intervention","label":"The tested mice regenerated cone pigment normally despite targeted DES1 loss.","description":"Müller-directed Degs1 deletion did not slow cone dark adaptation after greater than 90% pigment bleaching in isolated mouse retinas or intact eyes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0af2b309-dae6-5e4d-9d2c-4ac1faae585a","slug":"degs1-muller-loss","display_name":"Müller-directed DES1 loss","entity_type_key":"protein_state"},"role":"conditional perturbation","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"743b0a10-1977-5b02-a116-1c53c6700b3d","slug":"degs1","display_name":"Dihydroceramide desaturase 1 / DES1","entity_type_key":"protein"},"role":"targeted protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a4a3ca81-92c9-5e98-b325-7347e1a28b3e","slug":"cone-dark-adaptation","display_name":"Cone dark adaptation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 7C-D","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"505-nm ex vivo or 520-nm in vivo bleach; mutant versus heterozygous littermate controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not exclude other DES1 functions, residual extra-Müller activity, or species-specific contributions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested mice regenerated cone pigment normally despite targeted DES1 loss.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. (2019). https://pubmed.ncbi.nlm.nih.gov/30645148/ DOI: 10.1096/fj.201802493r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"neural retina and intact-eye recordings","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"685683d1-b114-5d5c-924f-177e3a57f1c6","evidence_kind":"source_excerpt","locator":"Lines 618-631","start_line":618,"end_line":631,"excerpt":"### vae-des1-deletion-cone-recovery\nMüller-directed Degs1 deletion did not slow cone dark adaptation after greater than 90% pigment bleaching in isolated mouse retinas or intact eyes.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The tested mice regenerated cone pigment normally despite targeted DES1 loss.\norganism: Mus musculus\ntissue_or_cell_type: neural retina and intact-eye recordings\nexperimental_model: Degs1-flox/Pdgfra-Cre conditional knockout on Gnat1-null background\nlimitations: Does not exclude other DES1 functions, residual extra-Müller activity, or species-specific contributions.\nexposure: 505-nm ex vivo or 520-nm in vivo bleach; mutant versus heterozygous littermate controls.\ncross_nutrient: false\nevidence_location: Figure 7C-D\nnutrient: Vitamin A\n[vav-kiser2019] Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones. 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