Component

Cellular molybdate uptake

Cellular molybdate uptake. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Reducing MFSD5 mRNA to 11-35% of control did not reduce molybdate uptake signals at the tested doses.

    Human MFSD5 / HsMOT2 → Cellular molybdate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 23464, "end_char": 24197, "text_sha256": "442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6"}
    experimental_model
    MolyProbe live-cell FRET, MFSD5 overexpression and siRNA
    exposure
    Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons
    limitations
    FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Homo sapiens
    plain_language
    This protein was not required for most measured uptake under these conditions.
    primary_references
    [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    tissue_or_cell_type
    HEK-293T cells

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 235–246

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · MolyProbe live-cell FRET, MFSD5 overexpression and siRNA · source_derived_draft · unverified_draft

    ### mo-mfsd5-knockdown-null Reducing MFSD5 mRNA to 11-35% of control did not reduce molybdate uptake signals at the tested doses. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: This protein was not required for most measured uptake under these conditions. organism: Homo sapiens tissue_or_cell_type: HEK-293T cells experimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA limitations: FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion. exposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons evidence_span: {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 23464, "end_char": 24197, "text_sha256": "442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6"} [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    Complete structured claim and evidence

  2. Large HsMOT2/MFSD5 overexpression increased the molybdate-sensitive FRET signal in HEK-293T cells.

    Human MFSD5 / HsMOT2 → Cellular molybdate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 23464, "end_char": 24197, "text_sha256": "442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6"}
    experimental_model
    MolyProbe live-cell FRET, MFSD5 overexpression and siRNA
    exposure
    Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons
    limitations
    The text reports millimolar values in this passage that differ from micromolar labeling elsewhere; this claim preserves the directional finding without inferring an exact effective concentration.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Homo sapiens
    plain_language
    Making much more of the transporter increased uptake in this cell model.
    primary_references
    [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    tissue_or_cell_type
    HEK-293T cells

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 222–233

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · MolyProbe live-cell FRET, MFSD5 overexpression and siRNA · source_derived_draft · unverified_draft

    ### mo-mfsd5-overexpression Large HsMOT2/MFSD5 overexpression increased the molybdate-sensitive FRET signal in HEK-293T cells. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: Making much more of the transporter increased uptake in this cell model. organism: Homo sapiens tissue_or_cell_type: HEK-293T cells experimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA limitations: The text reports millimolar values in this passage that differ from micromolar labeling elsewhere; this claim preserves the directional finding without inferring an exact effective concentration. exposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons evidence_span: {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 23464, "end_char": 24197, "text_sha256": "442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6"} [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    Complete structured claim and evidence

  3. Oxalate at 10 mM inhibited molybdate accumulation in HEK-293T cells.

    Oxalate → Cellular molybdate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 21554, "end_char": 22593, "text_sha256": "79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c"}
    experimental_model
    MolyProbe live-cell FRET, MFSD5 overexpression and siRNA
    exposure
    Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons
    limitations
    High experimental concentration; does not establish dietary oxalate causing human molybdenum deficiency.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Homo sapiens
    plain_language
    High oxalate inhibited entry in this laboratory assay.
    primary_references
    [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    tissue_or_cell_type
    HEK-293T cells

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 248–259

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · MolyProbe live-cell FRET, MFSD5 overexpression and siRNA · source_derived_draft · unverified_draft

    ### mo-oxalate-uptake Oxalate at 10 mM inhibited molybdate accumulation in HEK-293T cells. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: High oxalate inhibited entry in this laboratory assay. organism: Homo sapiens tissue_or_cell_type: HEK-293T cells experimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA limitations: High experimental concentration; does not establish dietary oxalate causing human molybdenum deficiency. exposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons evidence_span: {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 21554, "end_char": 22593, "text_sha256": "79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c"} [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    Complete structured claim and evidence

  4. Adding 1 mM sulfate had little effect on HEK-293T molybdate uptake signals.

    Sulfate / SO4(2-) → Cellular molybdate uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 21554, "end_char": 22593, "text_sha256": "79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c"}
    experimental_model
    MolyProbe live-cell FRET, MFSD5 overexpression and siRNA
    exposure
    Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons
    limitations
    FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.
    nutrient_topic
    Molybdenum research collection; topical membership is not evidence of a direct dietary effect. · Molybdenum
    organism
    Homo sapiens
    plain_language
    Sulfate did not strongly block this uptake route.
    primary_references
    [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    tissue_or_cell_type
    HEK-293T cells

    Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17) · lines 261–272

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · MolyProbe live-cell FRET, MFSD5 overexpression and siRNA · source_derived_draft · unverified_draft

    ### mo-sulfate-uptake-null Adding 1 mM sulfate had little effect on HEK-293T molybdate uptake signals. Condition category: normal nutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect. plain_language: Sulfate did not strongly block this uptake route. organism: Homo sapiens tissue_or_cell_type: HEK-293T cells experimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA limitations: FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion. exposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons evidence_span: {"source_cache": "artifacts/molybdenum-research/23472155.fulltext.txt", "locator": "Exact primary full-text span; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f", "start_char": 21554, "end_char": 22593, "text_sha256": "79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c"} [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards