{"id":"690ec183-7fe6-52a6-a767-e89665b330d8","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfate-uptake-null","predicate":"has_little_effect_in_cell_assay","statement":"Adding 1 mM sulfate had little effect on HEK-293T molybdate uptake signals.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"dbbb5e83-ee0f-5f66-80aa-195e18a89240","mechanism_event_label":"Sulfate did not strongly block this uptake route.","subject":{"id":"3634af03-823b-584f-8e8e-e6ab6e3fbb60","slug":"sulfate","display_name":"Sulfate / SO4(2-)","entity_type_key":"ion"},"object":{"id":"c864518b-3b61-5ee7-b361-d9148fac878c","slug":"cellular-molybdate-uptake","display_name":"Cellular molybdate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dbbb5e83-ee0f-5f66-80aa-195e18a89240","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfate-uptake-null-event","event_type":"biochemical_relationship","label":"Sulfate did not strongly block this uptake route.","description":"Adding 1 mM sulfate had little effect on HEK-293T molybdate uptake signals.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3634af03-823b-584f-8e8e-e6ab6e3fbb60","slug":"sulfate","display_name":"Sulfate / SO4(2-)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c864518b-3b61-5ee7-b361-d9148fac878c","slug":"cellular-molybdate-uptake","display_name":"Cellular molybdate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/23472155.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f\", \"start_char\": 21554, \"end_char\": 22593, \"text_sha256\": \"79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MolyProbe live-cell FRET, MFSD5 overexpression and siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Sulfate did not strongly block this uptake route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK-293T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9871509d-bea9-58b2-90f0-2f34c01a54a5","evidence_kind":"source_excerpt","locator":"Lines 261-272","start_line":261,"end_line":272,"excerpt":"### mo-sulfate-uptake-null\nAdding 1 mM sulfate had little effect on HEK-293T molybdate uptake signals.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Sulfate did not strongly block this uptake route.\norganism: Homo sapiens\ntissue_or_cell_type: HEK-293T cells\nexperimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA\nlimitations: FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.\nexposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23472155.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f\", \"start_char\": 21554, \"end_char\": 22593, \"text_sha256\": \"79bbaf42eff93e67e445d38383c53bbd3cea5b18c2e2afee38803b40f9b5fc4c\"}\n[mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. 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