{"id":"d2065921-d9ac-544a-a411-d982b4a20185","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mfsd5-knockdown-null","predicate":"knockdown_did_not_lower","statement":"Reducing MFSD5 mRNA to 11-35% of control did not reduce molybdate uptake signals at the tested doses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f423cf6c-145e-595f-a309-a785c1922803","mechanism_event_label":"This protein was not required for most measured uptake under these conditions.","subject":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"object":{"id":"c864518b-3b61-5ee7-b361-d9148fac878c","slug":"cellular-molybdate-uptake","display_name":"Cellular molybdate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f423cf6c-145e-595f-a309-a785c1922803","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mfsd5-knockdown-null-event","event_type":"biochemical_relationship","label":"This protein was not required for most measured uptake under these conditions.","description":"Reducing MFSD5 mRNA to 11-35% of control did not reduce molybdate uptake signals at the tested doses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49b228c5-a2e8-56a3-82a9-de12dd6f3731","slug":"mfsd5","display_name":"Human MFSD5 / HsMOT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c864518b-3b61-5ee7-b361-d9148fac878c","slug":"cellular-molybdate-uptake","display_name":"Cellular molybdate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/23472155.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f\", \"start_char\": 23464, \"end_char\": 24197, \"text_sha256\": \"442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MolyProbe live-cell FRET, MFSD5 overexpression and siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This protein was not required for most measured uptake under these conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK-293T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e61d889d-470e-5494-9743-dd0d988233ef","evidence_kind":"source_excerpt","locator":"Lines 235-246","start_line":235,"end_line":246,"excerpt":"### mo-mfsd5-knockdown-null\nReducing MFSD5 mRNA to 11-35% of control did not reduce molybdate uptake signals at the tested doses.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This protein was not required for most measured uptake under these conditions.\norganism: Homo sapiens\ntissue_or_cell_type: HEK-293T cells\nexperimental_model: MolyProbe live-cell FRET, MFSD5 overexpression and siRNA\nlimitations: FRET reports accessible molybdate, not total cofactor occupancy; mRNA knockdown does not prove complete protein depletion.\nexposure: Molybdate dose/time courses; 10 mM oxalate and 1 mM sulfate comparisons\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23472155.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a3c76154f22db125e0e3c74c1dadbb32edae742411e28763ce2116dfd2e8202f\", \"start_char\": 23464, \"end_char\": 24197, \"text_sha256\": \"442fa593fda3c65b9c6391a3030401fd13548679459d4ccd58ea4da88d9fdfc6\"}\n[mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175","model_system":"MolyProbe live-cell FRET, MFSD5 overexpression and siRNA","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p23472155] Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor. (2013). https://pubmed.ncbi.nlm.nih.gov/23472155/ DOI: 10.1371/journal.pone.0058175","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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