Component
Saccharomyces cerevisiae Nfs1–Isd11 complex
Saccharomyces cerevisiae Nfs1–Isd11 complex. Identity is distinct from its gene and experimentally modified states; see each claim for organism and scope.
3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Conditional Acp1 depletion in yeast reduced assembled Nfs1–Isd11 and left the remaining Nfs1 and Isd11 predominantly in insoluble aggregates.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- true
- evidence_location
- Full text line 117; Fig. 3A–B
- experimental_model
- TetO7-ACP1 conditional depletion and mitochondrial fractionation
- exposure
- 10 micrograms/mL doxycycline for 18 hours to suppress ACP1.
- limitations
- Loss of cysteine-desulfurase activity was a mechanistic inference from complex destabilization, not a direct rate claim in this record. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.
- nutrient_topic
- Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. · Pantothenate (vitamin B5)
- organism
- Saccharomyces cerevisiae
- plain_language
- Removing the carrier destabilized the sulfur-handling protein complex.
- primary_references
- [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
- tissue_or_cell_type
- Mitochondria
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17) · lines 879–891
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · TetO7-ACP1 conditional depletion and mitochondrial fractionation · source_derived_draft · unverified_draft
### b5-met-yeast-acp-complex-stability Conditional Acp1 depletion in yeast reduced assembled Nfs1–Isd11 and left the remaining Nfs1 and Isd11 predominantly in insoluble aggregates. Condition category: machinery_impairment nutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. plain_language: Removing the carrier destabilized the sulfur-handling protein complex. organism: Saccharomyces cerevisiae tissue_or_cell_type: Mitochondria experimental_model: TetO7-ACP1 conditional depletion and mitochondrial fractionation limitations: Loss of cysteine-desulfurase activity was a mechanistic inference from complex destabilization, not a direct rate claim in this record. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation. exposure: 10 micrograms/mL doxycycline for 18 hours to suppress ACP1. cross_nutrient: true evidence_location: Full text line 117; Fig. 3A–B [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
Complete structured claim and evidenceEndogenous yeast Acp1 copurified with Nfs1, Isd11 and Isu1; reciprocal immunoprecipitation confirmed association with the Nfs1–Isd11 core.
Experimental context and source evidence
- cross_nutrient
- true
- evidence_location
- Full text lines 73; Fig. 1B and Fig. 1 supplement 3
- experimental_model
- Tagged endogenous protein immunoprecipitation and blue-native PAGE
- exposure
- Functional endogenous tagged proteins, detergent-solubilized mitochondria.
- limitations
- Stable association does not by itself quantify sulfur transfer; the protein identities are yeast, not human. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.
- nutrient_topic
- Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. · Pantothenate (vitamin B5)
- organism
- Saccharomyces cerevisiae
- plain_language
- The mitochondrial carrier physically associates with the iron–sulfur assembly machinery.
- primary_references
- [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
- tissue_or_cell_type
- Isolated mitochondria
Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17) · lines 865–877
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Tagged endogenous protein immunoprecipitation and blue-native PAGE · source_derived_draft · unverified_draft
### b5-met-yeast-acp-isu-binding Endogenous yeast Acp1 copurified with Nfs1, Isd11 and Isu1; reciprocal immunoprecipitation confirmed association with the Nfs1–Isd11 core. Condition category: normal nutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. plain_language: The mitochondrial carrier physically associates with the iron–sulfur assembly machinery. organism: Saccharomyces cerevisiae tissue_or_cell_type: Isolated mitochondria experimental_model: Tagged endogenous protein immunoprecipitation and blue-native PAGE limitations: Stable association does not by itself quantify sulfur transfer; the protein identities are yeast, not human. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation. exposure: Functional endogenous tagged proteins, detergent-solubilized mitochondria. cross_nutrient: true evidence_location: Full text lines 73; Fig. 1B and Fig. 1 supplement 3 [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
Complete structured claim and evidenceThe yeast Acp1 S82A variant, which cannot carry phosphopantetheine at that site, only modestly restored Nfs1–Isd11 abundance in Acp1-depleted cells, unlike wild-type Acp1.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- true
- evidence_location
- Full text line 151; Fig. 4A–B
- experimental_model
- Wild-type versus S82A rescue of conditional yeast ACP1 depletion
- exposure
- Plasmid expression; 18 hours of 10 micrograms/mL doxycycline suppression.
- limitations
- S82A retained viability and some aconitase rescue. The study did not directly prove identical folding of apo and holo carrier proteins. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.
- nutrient_topic
- Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. · Pantothenate (vitamin B5)
- organism
- Saccharomyces cerevisiae
- plain_language
- The protein without its CoA-derived arm retained some function, but failed to restore normal complex stability.
- primary_references
- [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
- tissue_or_cell_type
- Mitochondria
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17) · lines 893–905
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Wild-type versus S82A rescue of conditional yeast ACP1 depletion · source_derived_draft · unverified_draft
### b5-met-yeast-acp-s82a-partial The yeast Acp1 S82A variant, which cannot carry phosphopantetheine at that site, only modestly restored Nfs1–Isd11 abundance in Acp1-depleted cells, unlike wild-type Acp1. Condition category: machinery_impairment nutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect. plain_language: The protein without its CoA-derived arm retained some function, but failed to restore normal complex stability. organism: Saccharomyces cerevisiae tissue_or_cell_type: Mitochondria experimental_model: Wild-type versus S82A rescue of conditional yeast ACP1 depletion limitations: S82A retained viability and some aconitase rescue. The study did not directly prove identical folding of apo and holo carrier proteins. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation. exposure: Plasmid expression; 18 hours of 10 micrograms/mL doxycycline suppression. cross_nutrient: true evidence_location: Full text line 151; Fig. 4A–B [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.