{"id":"ec295717-f03d-5148-bcdf-f2406f713b05","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-acp-complex-stability","predicate":"depletion_destabilizes","statement":"Conditional Acp1 depletion in yeast reduced assembled Nfs1–Isd11 and left the remaining Nfs1 and Isd11 predominantly in insoluble aggregates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"52d9c794-fa27-5707-8d17-8f16b9853306","mechanism_event_label":"Removing the carrier destabilized the sulfur-handling protein complex.","subject":{"id":"630ff358-f141-5262-891f-f902d1259d65","slug":"yeast-acp1","display_name":"Saccharomyces cerevisiae mitochondrial acyl carrier protein / Acp1","entity_type_key":"protein"},"object":{"id":"e4f52704-e22e-5945-b303-be76ec4c8a52","slug":"yeast-nfs1-isd11-complex","display_name":"Saccharomyces cerevisiae Nfs1–Isd11 complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"52d9c794-fa27-5707-8d17-8f16b9853306","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-acp-complex-stability-event","event_type":"biochemical_relationship","label":"Removing the carrier destabilized the sulfur-handling protein complex.","description":"Conditional Acp1 depletion in yeast reduced assembled Nfs1–Isd11 and left the remaining Nfs1 and Isd11 predominantly in insoluble aggregates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"76d1d7a1-077c-508d-8f8f-6f5ece005cfe","slug":"yeast-nfs1","display_name":"Saccharomyces cerevisiae cysteine desulfurase / Nfs1","entity_type_key":"protein"},"role":"complex_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89e8a0e2-957f-5cb2-8628-bac8fff81d05","slug":"yeast-isd11","display_name":"Saccharomyces cerevisiae Isd11","entity_type_key":"protein"},"role":"complex_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a51efa8-f029-59ac-9ec3-b83e5eaccd84","slug":"yeast-isu1","display_name":"Saccharomyces cerevisiae iron-sulfur assembly scaffold / Isu1","entity_type_key":"protein"},"role":"scaffold","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"carrier_prosthetic_group","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"630ff358-f141-5262-891f-f902d1259d65","slug":"yeast-acp1","display_name":"Saccharomyces cerevisiae mitochondrial acyl carrier protein / Acp1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"e4f52704-e22e-5945-b303-be76ec4c8a52","slug":"yeast-nfs1-isd11-complex","display_name":"Saccharomyces cerevisiae Nfs1–Isd11 complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text line 117; Fig. 3A–B","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"TetO7-ACP1 conditional depletion and mitochondrial fractionation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 micrograms/mL doxycycline for 18 hours to suppress ACP1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Loss of cysteine-desulfurase activity was a mechanistic inference from complex destabilization, not a direct rate claim in this record. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Saccharomyces cerevisiae","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing the carrier destabilized the sulfur-handling protein complex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1bee9120-97d5-5a35-9d4b-a91a91e2b398","evidence_kind":"source_excerpt","locator":"Lines 879-891","start_line":879,"end_line":891,"excerpt":"### b5-met-yeast-acp-complex-stability\nConditional Acp1 depletion in yeast reduced assembled Nfs1–Isd11 and left the remaining Nfs1 and Isd11 predominantly in insoluble aggregates.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing the carrier destabilized the sulfur-handling protein complex.\norganism: Saccharomyces cerevisiae\ntissue_or_cell_type: Mitochondria\nexperimental_model: TetO7-ACP1 conditional depletion and mitochondrial fractionation\nlimitations: Loss of cysteine-desulfurase activity was a mechanistic inference from complex destabilization, not a direct rate claim in this record. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: 10 micrograms/mL doxycycline for 18 hours to suppress ACP1.\ncross_nutrient: true\nevidence_location: Full text line 117; Fig. 3A–B\n[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","model_system":"TetO7-ACP1 conditional depletion and mitochondrial fractionation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. 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