{"id":"6d2893fa-361f-519f-b4d7-f5c6b7d7a870","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-acp-isu-binding","predicate":"associates_with","statement":"Endogenous yeast Acp1 copurified with Nfs1, Isd11 and Isu1; reciprocal immunoprecipitation confirmed association with the Nfs1–Isd11 core.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"1371353f-104d-588b-9822-a0613d471500","mechanism_event_label":"The mitochondrial carrier physically associates with the iron–sulfur assembly machinery.","subject":{"id":"630ff358-f141-5262-891f-f902d1259d65","slug":"yeast-acp1","display_name":"Saccharomyces cerevisiae mitochondrial acyl carrier protein / Acp1","entity_type_key":"protein"},"object":{"id":"e4f52704-e22e-5945-b303-be76ec4c8a52","slug":"yeast-nfs1-isd11-complex","display_name":"Saccharomyces cerevisiae Nfs1–Isd11 complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"1371353f-104d-588b-9822-a0613d471500","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-acp-isu-binding-event","event_type":"biochemical_relationship","label":"The mitochondrial carrier physically associates with the iron–sulfur assembly machinery.","description":"Endogenous yeast Acp1 copurified with Nfs1, Isd11 and Isu1; reciprocal immunoprecipitation confirmed association with the Nfs1–Isd11 core.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"76d1d7a1-077c-508d-8f8f-6f5ece005cfe","slug":"yeast-nfs1","display_name":"Saccharomyces cerevisiae cysteine desulfurase / Nfs1","entity_type_key":"protein"},"role":"complex_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89e8a0e2-957f-5cb2-8628-bac8fff81d05","slug":"yeast-isd11","display_name":"Saccharomyces cerevisiae Isd11","entity_type_key":"protein"},"role":"complex_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a51efa8-f029-59ac-9ec3-b83e5eaccd84","slug":"yeast-isu1","display_name":"Saccharomyces cerevisiae iron-sulfur assembly scaffold / Isu1","entity_type_key":"protein"},"role":"scaffold","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"carrier_prosthetic_group","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"630ff358-f141-5262-891f-f902d1259d65","slug":"yeast-acp1","display_name":"Saccharomyces cerevisiae mitochondrial acyl carrier protein / Acp1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"e4f52704-e22e-5945-b303-be76ec4c8a52","slug":"yeast-nfs1-isd11-complex","display_name":"Saccharomyces cerevisiae Nfs1–Isd11 complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 73; Fig. 1B and Fig. 1 supplement 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Tagged endogenous protein immunoprecipitation and blue-native PAGE","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Functional endogenous tagged proteins, detergent-solubilized mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Stable association does not by itself quantify sulfur transfer; the protein identities are yeast, not human. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Saccharomyces cerevisiae","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mitochondrial carrier physically associates with the iron–sulfur assembly machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Isolated mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dba01be5-93d1-5bda-afc7-b731adede811","evidence_kind":"source_excerpt","locator":"Lines 865-877","start_line":865,"end_line":877,"excerpt":"### b5-met-yeast-acp-isu-binding\nEndogenous yeast Acp1 copurified with Nfs1, Isd11 and Isu1; reciprocal immunoprecipitation confirmed association with the Nfs1–Isd11 core.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The mitochondrial carrier physically associates with the iron–sulfur assembly machinery.\norganism: Saccharomyces cerevisiae\ntissue_or_cell_type: Isolated mitochondria\nexperimental_model: Tagged endogenous protein immunoprecipitation and blue-native PAGE\nlimitations: Stable association does not by itself quantify sulfur transfer; the protein identities are yeast, not human. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Functional endogenous tagged proteins, detergent-solubilized mitochondria.\ncross_nutrient: true\nevidence_location: Full text lines 73; Fig. 1B and Fig. 1 supplement 3\n[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","model_system":"Tagged endogenous protein immunoprecipitation and blue-native PAGE","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}