Component
Sulforaphane-derived activity-based probe
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
The sulforaphane-derived activity-based probe captured STAT3 from BB3RC61 cells.
Experimental context and source evidence
- experimental_model
- Human antiestrogen-resistant metastatic breast cancer sample BB3RC61
- exposure
- 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.
- limitations
- The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.
- organism
- Human
- primary_locator
- Figure 4e; Supplementary Methods: Affinity pull-down assays.
- primary_references
- https://doi.org/10.1038/s41388-020-1335-z
APT2-STAT3 cycling and a competing sulforaphane target route · lines 87–93
Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z. · supports · Human antiestrogen-resistant metastatic breast cancer sample BB3RC61 · source_derived_draft · unverified_draft
The sulforaphane-derived activity-based probe captured STAT3 from BB3RC61 cells. primary_references: https://doi.org/10.1038/s41388-020-1335-z primary_locator: Figure 4e; Supplementary Methods: Affinity pull-down assays. organism: Human experimental_model: Human antiestrogen-resistant metastatic breast cancer sample BB3RC61 exposure: 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot. limitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.
Complete structured claim and evidenceThe sulforaphane-derived activity-based probe captured STAT3 from HBCX34 cells.
Experimental context and source evidence
- experimental_model
- Human HBCx34 breast cancer patient-derived xenograft tumor cells
- exposure
- 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.
- limitations
- The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.
- organism
- Human
- primary_locator
- Figure 4c; Supplementary Methods: Affinity pull-down assays.
- primary_references
- https://doi.org/10.1038/s41388-020-1335-z
APT2-STAT3 cycling and a competing sulforaphane target route · lines 78–84
Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z. · supports · Human HBCx34 breast cancer patient-derived xenograft tumor cells · source_derived_draft · unverified_draft
The sulforaphane-derived activity-based probe captured STAT3 from HBCX34 cells. primary_references: https://doi.org/10.1038/s41388-020-1335-z primary_locator: Figure 4c; Supplementary Methods: Affinity pull-down assays. organism: Human experimental_model: Human HBCx34 breast cancer patient-derived xenograft tumor cells exposure: 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot. limitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.