{"id":"f791da70-9574-5eb3-af9e-d973f9f75fd0","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfn-probe-stat3-bb3rc61","predicate":"has_contextual_experimental_result","statement":"The sulforaphane-derived activity-based probe captured STAT3 from BB3RC61 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"86acc014-7372-58c5-8f4a-69f5cc9afbe0","mechanism_event_label":"sfn-probe-stat3-bb3rc61","subject":{"id":"bc7a36bf-0330-5903-b21c-495f4059cc31","slug":"sulforaphane-activity-based-probe","display_name":"Sulforaphane-derived activity-based probe","entity_type_key":"chemical_species"},"object":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"86acc014-7372-58c5-8f4a-69f5cc9afbe0","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfn-probe-stat3-bb3rc61","event_type":"experimental_result","label":"sfn-probe-stat3-bb3rc61","description":"The sulforaphane-derived activity-based probe captured STAT3 from BB3RC61 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bc7a36bf-0330-5903-b21c-495f4059cc31","slug":"sulforaphane-activity-based-probe","display_name":"Sulforaphane-derived activity-based probe","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"parent compound of chemical probe","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human antiestrogen-resistant metastatic breast cancer sample BB3RC61","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 4e; Supplementary Methods: Affinity pull-down assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41388-020-1335-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8098c666-dd4e-5a19-a8fa-6fd3c1688111","evidence_kind":"source_excerpt","locator":"Lines 87-93","start_line":87,"end_line":93,"excerpt":"The sulforaphane-derived activity-based probe captured STAT3 from BB3RC61 cells.\nprimary_references: https://doi.org/10.1038/s41388-020-1335-z\nprimary_locator: Figure 4e; Supplementary Methods: Affinity pull-down assays.\norganism: Human\nexperimental_model: Human antiestrogen-resistant metastatic breast cancer sample BB3RC61\nexposure: 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.\nlimitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","model_system":"Human antiestrogen-resistant metastatic breast cancer sample BB3RC61","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the specified primary result; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01fc674f-07c6-58fd-b1e7-877478a4472e","stable_key":"import-708c252b-0aef-5cec-95bf-99d19578214c","title":"APT2-STAT3 cycling and a competing sulforaphane target route","document_type":"imported_text","citation_label":"Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z.","file_path":"","sha256":"b1b245468dac7acc94a2fef690d994124b2bc4fc5f92135173cf5636af391395","revision_id":"d84b8419-69dd-50d9-b587-5627ed414591","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}