{"id":"316378bc-e7b1-5908-86d3-90eb59461efc","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfn-probe-stat3-hbcx34","predicate":"has_contextual_experimental_result","statement":"The sulforaphane-derived activity-based probe captured STAT3 from HBCX34 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0502f4dc-89fa-5d6a-a55a-062063a272ea","mechanism_event_label":"sfn-probe-stat3-hbcx34","subject":{"id":"bc7a36bf-0330-5903-b21c-495f4059cc31","slug":"sulforaphane-activity-based-probe","display_name":"Sulforaphane-derived activity-based probe","entity_type_key":"chemical_species"},"object":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0502f4dc-89fa-5d6a-a55a-062063a272ea","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:sfn-probe-stat3-hbcx34","event_type":"experimental_result","label":"sfn-probe-stat3-hbcx34","description":"The sulforaphane-derived activity-based probe captured STAT3 from HBCX34 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bc7a36bf-0330-5903-b21c-495f4059cc31","slug":"sulforaphane-activity-based-probe","display_name":"Sulforaphane-derived activity-based probe","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"parent compound of chemical probe","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human HBCx34 breast cancer patient-derived xenograft tumor cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 4c; Supplementary Methods: Affinity pull-down assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41388-020-1335-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dc1ce193-803a-5de6-a9ba-1572dbf793f1","evidence_kind":"source_excerpt","locator":"Lines 78-84","start_line":78,"end_line":84,"excerpt":"The sulforaphane-derived activity-based probe captured STAT3 from HBCX34 cells.\nprimary_references: https://doi.org/10.1038/s41388-020-1335-z\nprimary_locator: Figure 4c; Supplementary Methods: Affinity pull-down assays.\norganism: Human\nexperimental_model: Human HBCx34 breast cancer patient-derived xenograft tumor cells\nexposure: 5 micromolar SFN-ABP for 30 minutes at 37 C in serum-free DMEM; click chemistry and avidin enrichment followed by anti-STAT3 immunoblot.\nlimitations: The chemical probe and stabilized formulation are separate experimental actors. Capture supports engagement but does not identify the modified STAT3 cysteine or demonstrate that APT2 mediates the effect. No GPX4 time-course endpoint. Primary cell/PDX studies are not evidence of benefit from dietary broccoli.","model_system":"Human HBCx34 breast cancer patient-derived xenograft tumor cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the specified primary result; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01fc674f-07c6-58fd-b1e7-877478a4472e","stable_key":"import-708c252b-0aef-5cec-95bf-99d19578214c","title":"APT2-STAT3 cycling and a competing sulforaphane target route","document_type":"imported_text","citation_label":"Selected primary observations: 10.1038/s41586-020-2799-2 and 10.1038/s41388-020-1335-z.","file_path":"","sha256":"b1b245468dac7acc94a2fef690d994124b2bc4fc5f92135173cf5636af391395","revision_id":"d84b8419-69dd-50d9-b587-5627ed414591","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}