Mammalian mitochondrial sodium/calcium exchanger NCLX / SLC8B1: molecular mechanisms & dependencies

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

Human evidence only

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

Activation of mitochondrial sodium/calcium exchange promoted sodium import into the matrix during the hypoxic pathway.
Mammalian mitochondrial sodium/calcium exchanger NCLX / SLC8B1 → Sodium import into the mitochondrial matrix source_derived_draftungraded
Experimental context and source evidence
evidence_span
{"source_cache": "artifacts/sodium-research/32728214.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1", "start_char": 0, "end_char": 1533, "text_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1"}
experimental_model
Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays
exposure
Acute hypoxia and inhibition of mitochondrial sodium/calcium exchange
limitations
Mechanistic research under hypoxia, including multiple preparations. Do not infer that dietary salt drives this chain, that every tissue responds identically, or that ROS here proves disease.
nutrient_topic
Sodium research collection; topical membership is not evidence of a direct dietary effect. · Sodium
organism
Human and mouse cells; additional rat vascular experiments in the paper
plain_language
Calcium handling can bring sodium into the mitochondrion.
primary_references
[sodium-p32728214] Na+ controls hypoxic signalling by the mitochondrial respiratory chain. (2020). https://pubmed.ncbi.nlm.nih.gov/32728214/ DOI: 10.1038/s41586-020-2551-y
tissue_or_cell_type
Mitochondrial matrix and inner membrane
Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17) · lines 772–783
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays · source_derived_draft · unverified_draft
### sodium-matrix-sodium Activation of mitochondrial sodium/calcium exchange promoted sodium import into the matrix during the hypoxic pathway. Condition category: normal nutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect. plain_language: Calcium handling can bring sodium into the mitochondrion. organism: Human and mouse cells; additional rat vascular experiments in the paper tissue_or_cell_type: Mitochondrial matrix and inner membrane experimental_model: Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays limitations: Mechanistic research under hypoxia, including multiple preparations. Do not infer that dietary salt drives this chain, that every tissue responds identically, or that ROS here proves disease. exposure: Acute hypoxia and inhibition of mitochondrial sodium/calcium exchange evidence_span: {"source_cache": "artifacts/sodium-research/32728214.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1", "start_char": 0, "end_char": 1533, "text_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1"} [sodium-p32728214] Na+ controls hypoxic signalling by the mitochondrial respiratory chain. (2020). https://pubmed.ncbi.nlm.nih.gov/32728214/ DOI: 10.1038/s41586-020-2551-y
Complete structured claim and evidence
NCLX overexpression increased sodium-dependent mitochondrial calcium efflux; silencing reduced it and heterologous rescue restored it.
Mammalian mitochondrial sodium/calcium exchanger NCLX / SLC8B1 → Mitochondrial calcium efflux source_derived_draftungraded
Experimental context and source evidence
evidence_span
{"source_cache": "artifacts/sodium-research/20018762.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8594465b44cbb04ed3a631d5dae19959e7c0494a86537d6a60d33d6db1821831", "start_char": 0, "end_char": 1085, "text_sha256": "8594465b44cbb04ed3a631d5dae19959e7c0494a86537d6a60d33d6db1821831"}
experimental_model
Overexpression, siRNA, rescue, mutant and fluorescent ion-imaging experiments
exposure
NCLX abundance, inactive mutant and ion substitutions
limitations
Supports molecular identification in these assays. Protein species and construct details must be taken from the original methods; not evidence for raising dietary sodium.
nutrient_topic
Sodium research collection; topical membership is not evidence of a direct dietary effect. · Sodium
organism
Mammalian cultured-cell preparations
plain_language
A mitochondrial exchanger connects sodium movement with calcium removal.
primary_references
[sodium-p20018762] NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. (2010). https://pubmed.ncbi.nlm.nih.gov/20018762/ DOI: 10.1073/pnas.0908099107
tissue_or_cell_type
Mitochondrial cristae
Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17) · lines 733–744
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Overexpression, siRNA, rescue, mutant and fluorescent ion-imaging experiments · source_derived_draft · unverified_draft
### sodium-nclx-efflux NCLX overexpression increased sodium-dependent mitochondrial calcium efflux; silencing reduced it and heterologous rescue restored it. Condition category: normal nutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect. plain_language: A mitochondrial exchanger connects sodium movement with calcium removal. organism: Mammalian cultured-cell preparations tissue_or_cell_type: Mitochondrial cristae experimental_model: Overexpression, siRNA, rescue, mutant and fluorescent ion-imaging experiments limitations: Supports molecular identification in these assays. Protein species and construct details must be taken from the original methods; not evidence for raising dietary sodium. exposure: NCLX abundance, inactive mutant and ion substitutions evidence_span: {"source_cache": "artifacts/sodium-research/20018762.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "8594465b44cbb04ed3a631d5dae19959e7c0494a86537d6a60d33d6db1821831", "start_char": 0, "end_char": 1085, "text_sha256": "8594465b44cbb04ed3a631d5dae19959e7c0494a86537d6a60d33d6db1821831"} [sodium-p20018762] NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. (2010). https://pubmed.ncbi.nlm.nih.gov/20018762/ DOI: 10.1073/pnas.0908099107
Complete structured claim and evidence

Where it participates (unsigned role)

The pathway produced complex III superoxide; inhibiting mitochondrial sodium import blocked the response.
Sodium ion → Complex III superoxide production source_derived_draftungraded
Experimental context and source evidence
evidence_span
{"source_cache": "artifacts/sodium-research/32728214.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1", "start_char": 0, "end_char": 1533, "text_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1"}
experimental_model
Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays
exposure
Acute hypoxia and inhibition of mitochondrial sodium/calcium exchange
limitations
Mechanistic research under hypoxia, including multiple preparations. Do not infer that dietary salt drives this chain, that every tissue responds identically, or that ROS here proves disease.
nutrient_topic
Sodium research collection; topical membership is not evidence of a direct dietary effect. · Sodium
organism
Human and mouse cells; additional rat vascular experiments in the paper
plain_language
A sodium-linked membrane effect contributed to a mitochondrial redox signal.
primary_references
[sodium-p32728214] Na+ controls hypoxic signalling by the mitochondrial respiratory chain. (2020). https://pubmed.ncbi.nlm.nih.gov/32728214/ DOI: 10.1038/s41586-020-2551-y
tissue_or_cell_type
Mitochondrial matrix and inner membrane
Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17) · lines 811–822
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays · source_derived_draft · unverified_draft
### sodium-complex3-ros The pathway produced complex III superoxide; inhibiting mitochondrial sodium import blocked the response. Condition category: normal nutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect. plain_language: A sodium-linked membrane effect contributed to a mitochondrial redox signal. organism: Human and mouse cells; additional rat vascular experiments in the paper tissue_or_cell_type: Mitochondrial matrix and inner membrane experimental_model: Acute-hypoxia cellular and mitochondrial experiments with ion imaging and respiratory/membrane assays limitations: Mechanistic research under hypoxia, including multiple preparations. Do not infer that dietary salt drives this chain, that every tissue responds identically, or that ROS here proves disease. exposure: Acute hypoxia and inhibition of mitochondrial sodium/calcium exchange evidence_span: {"source_cache": "artifacts/sodium-research/32728214.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1", "start_char": 0, "end_char": 1533, "text_sha256": "2780431aa362c99c816f986e923fd65ed0c0324e8c8674af8ff2a2f9367762c1"} [sodium-p32728214] Na+ controls hypoxic signalling by the mitochondrial respiratory chain. (2020). https://pubmed.ncbi.nlm.nih.gov/32728214/ DOI: 10.1038/s41586-020-2551-y
Complete structured claim and evidence

The events it takes part in

A mechanism often involves more than two components. These are the full events, with every participant and its role.

Situations it appears in

Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

Open hypotheses

Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.

This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

Mammalian mitochondrial sodium/calcium exchanger NCLX / SLC8B1

How nutrients influence it

Other things that act on it