Component
SH-SY5Y cell viability by MTT
SH-SY5Y cell viability by MTT
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Pyridoxine reduced MTT-measured viability of differentiated SH-SY5Y cells after 24 hours in a concentration-dependent manner.
Experimental context and source evidence
- experimental_model
- Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays
- exposure
- Pyridoxine 0-5 micromolar for 24 hours.
- exposure-class
- Experimental excess pyridoxine; not dietary B6 deficiency · Pyridoxine
- limitations
- Tumor-derived cells and MTT endpoint; no oral-dose conversion or human toxicity threshold.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Homo sapiens
- plain_language
- Added pyridoxine harmed this experimental neuronal cell model.
- primary_references
- [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009
- tissue_or_cell_type
- Differentiated SH-SY5Y neuroblastoma cells
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1232–1243
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays · source_derived_draft · unverified_draft
### b6-neuro-pn-cell-viability Pyridoxine reduced MTT-measured viability of differentiated SH-SY5Y cells after 24 hours in a concentration-dependent manner. Condition category: normal nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Added pyridoxine harmed this experimental neuronal cell model. organism: Homo sapiens tissue_or_cell_type: Differentiated SH-SY5Y neuroblastoma cells experimental_model: Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays limitations: Tumor-derived cells and MTT endpoint; no oral-dose conversion or human toxicity threshold. exposure: Pyridoxine 0-5 micromolar for 24 hours. exposure-class: Experimental excess pyridoxine; not dietary B6 deficiency [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009
Complete structured claim and evidencePyridoxal, pyridoxamine, PLP and PMP did not reduce viability in the tested 24-hour cell assay.
Experimental context and source evidence
- experimental_model
- Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays
- exposure
- Comparators 0-500 micromolar for 24 hours.
- exposure-class
- Experimental excess pyridoxine; not dietary B6 deficiency · Pyridoxine
- limitations
- A negative short-term cell assay proves neither universal safety nor equivalent toxicity.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Homo sapiens
- plain_language
- The tested B6 forms behaved differently in this assay.
- primary_references
- [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009
- tissue_or_cell_type
- SH-SY5Y and CaCo-2 cells
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1245–1256
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays · source_derived_draft · unverified_draft
### b6-neuro-pn-vitamer-specificity Pyridoxal, pyridoxamine, PLP and PMP did not reduce viability in the tested 24-hour cell assay. Condition category: normal nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: The tested B6 forms behaved differently in this assay. organism: Homo sapiens tissue_or_cell_type: SH-SY5Y and CaCo-2 cells experimental_model: Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays limitations: A negative short-term cell assay proves neither universal safety nor equivalent toxicity. exposure: Comparators 0-500 micromolar for 24 hours. exposure-class: Experimental excess pyridoxine; not dietary B6 deficiency [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.